ABCMdb

PMID: 16410345

Kunzelmann K, Scheidt K, Scharf B, Ousingsawat J, Schreiber R, Wainwright B, McMorran B
Flagellin of Pseudomonas aeruginosa inhibits Na+ transport in airway epithelia.
FASEB J. 2006 Mar;20(3):545-6. Epub 2006 Jan 12., [PubMed]

Sentences

No. Mutations Sentence Comment

7 ABCC7 p.Gly551Asp Inhibition of ENaC was observed in tracheas of wild-type mice and was attenuated in mice homozygous for the frequent cystic fibrosis conductance regulator (CFTR) mutation G551D. Login to comment 42 ABCC7 p.Gly551Asp Mice, homozygous for the Cftr-G551D mutation (CF), or wild-type strain C57BL/6 (Charles River Laboratories, Wilmington, MA) were infected with P. aeruginosa using the agar bead protocol described in detail in McMorran et al. (20). Login to comment 82 ABCC7 p.Gly551Asp Here we examined ASGM1 expression in airway epithelial cells of tracheas from both wild-type mice and mice homozygous for the common CFTR mutation G551D (CF mice). Login to comment 95 ABCC7 p.Gly551Asp Effects of flagellin in CF mouse tracheas and tracheas from P. aeruginosa-infected mice Similar experiments were performed on tracheas from transgenic mice homozygous for the frequent CFTR mutation G551D (24). Login to comment 96 ABCC7 p.Gly551Asp Tracheas from cftrG551D/G551D mice demonstrated a spontaneous transepithelial voltage of -16.2 ± 1.9 mV, a calculated Isc of -292.2 ± 26.1 µA/cm2 , and a transepithelial resistance of 55.2 ± 4.1 Ωcm2 (n=5), which is consistent with previous recordings made with this mouse strain. Login to comment 151 ABCC7 p.Gly551Asp We found reduced inhibition of amiloride-sensitive Na+ absorption by flagellin of P. aeruginosa in the cftrG551D/G551D mice, lacking functional CFTR. Login to comment 152 ABCC7 p.Gly551Asp This remains currently unexplained since expression of membrane ASGM1 receptors appeared not reduced in cftrG551D/G551D mice when compared with wild-type animals. Login to comment 154 ABCC7 p.Gly551Asp We demonstrated previously that enhanced P. aeruginosa burden in the airways of cftrG551D/G551D mice was corrected by overexpression of wild-type CFTR, which enhanced fluid transport and normalized pathogen clearance (36). Login to comment