ABCMdb

PMID: 16107775

Nishioka C, Sakaeda T, Nakamura T, Moriya Y, Okamura N, Tamura T, Nakahara T, Aoyama N, Kamigaki T, Ohno M, Kuroda Y, Kasuga M, Okumura K
MDR1, MRP1 and MRP2 genotypes and in vitro chemosensitivity in Japanese patients with colorectal adenocarcinomas.
Kobe J Med Sci. 2004;50(5-6):181-8., [PubMed]

Sentences

No. Mutations Sentence Comment

4 ABCB1 p.Gly1249Ala In this study, 3 genotypes of MDR1, 4 genotypes of MRP1, and 6 genotypes of MRP2 were additionally evaluated, and it was suggested that MDR1 C3435T and MRP2 G1249A were related with the susceptibility to colorectal adenocarcinoma. Login to comment 19 ABCB1 p.Gly1249Ala In the present study, the distributions of MDR1 genotypes of T-129C, G2677(A,T) and C3435T, MRP1 genotypes of G128C, C218T, G2168A and G3173A, and MRP2 genotypes of C-24T, G1249A, C2302T, C2366T, C3972T and G4348A were evaluated in Japanese patients with primary colorectal adenocarcinoma, and their effects on the chemosensitivity against 5-FU, SN-38, MMC and CDDP, and also on the growth rate of tumor and mRNA expression levels of MDR1, MRP1 and MRP2 in the adenocarcinoma were examined for future individualization of cancer chemotherapy based on the genotyping of these transporters. Login to comment 37 ABCB1 p.Gly1249Ala The polymorphism of T-129C in the promoter region, a missense polymorphism of G2677(A,T), and a silent polymorphism of C3435T in the MDR1 gene, 4 missense polymorphisms of G128C, C218T, G2168A and G3173A in the MRP1 gene, and a polymorphism of C-24T in the 5`-flanking region, 4 missense polymorphisms of G1249A, C2302T, C2366T and G4348A, and a silent polymorphism of C3972T in the MRP2 gene were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and confirmed by direct sequencing. Login to comment 45 ABCB1 p.Gly1249Cys In addition, Ito et al., have reported on MRP2 G1249C position, showing that GG/GA/AA was 37/10/1 in genotype and G/A was 84/12 in allele frequency. Login to comment 46 ABCB1 p.Gly1249Ala Compared with the healthy subjects, T-allele at C3435T of MDR1 was more frequently found in patients with colorectal adenocarcinoma, whereas A-allele at G1249A of MRP2 was found less frequently, although this is from a small number of patients with insufficient statistical power. Login to comment 51 ABCB1 p.Gly1249Ala MDR1, MRP1 and MRP2 genotypes in 13 Japanese patients with colorectal adenocarcinomas Genotype Allele Position w/w w/m m/m w m MDR1 T-129C 11 2 0 24 2 G2677(A,T) 3 6 4 12 14 C3435T 3 6 4 12 14 MRP1 G128C 13 0 0 26 0 C218T 13 0 0 26 0 G2168A 10 3 0 23 3 G3173A 13 0 0 26 0 MRP2 C-24T 7 4 2 18 8 G1249A 12 1 0 25 1 C2302T 13 0 0 26 0 C2366T 13 0 0 26 0 C3972T 7 4 2 18 8 G4348A 13 0 0 26 0 The symbols "w" and "m" mean the polymorphisms with higher and lower frequency of allele, respectively. Login to comment 66 ABCB1 p.Gly1249Ala ABCB1 p.Gly1249Ala In conclusion, MDR1 genotypes of T-129C, G2677(A,T) and C3435T, MRP1 genotypes of G128C, C218T, G2168A and G3173A, and MRP2 genotypes of C-24T, G1249A, C2302T, C2366T, C3972T and G4348A were evaluated in the Japanese patients with primary colorectal adenocarcinoma, and it was suggested that MDR1 C3435T and MRP2 G1249A were related with the susceptibility to colorectal adenocarcinoma. Login to comment