ABCMdb

PMID: 15821043

Meyer zu Schwabedissen HE, Jedlitschky G, Gratz M, Haenisch S, Linnemann K, Fusch C, Cascorbi I, Kroemer HK
Variable expression of MRP2 (ABCC2) in human placenta: influence of gestational age and cellular differentiation.
Drug Metab Dispos. 2005 Jul;33(7):896-904. Epub 2005 Apr 8., [PubMed]

Sentences

No. Mutations Sentence Comment

35 ABCC5 p.Gly1249Ala In this paper we demonstrate a significant reduced expression in preterm placenta, increased expression during in vitro differentiation, and an influence of the G1249A polymorphism. Login to comment 120 ABCC5 p.Gly1249Ala These primers were used for amplification of a 301-bp fragment in the 5Ј-flanking region containing the C-24T variant of a 260-bp fragment in exon 10 containing the G1249A variant, and of a 184-bp fragment in exon 28 containing the 3972 polymorphism. Login to comment 186 ABCC5 p.Gly1249Ala Moreover, 5 homozygotes and 16 heterozygotes of the missense mutation G1249A (exon 10) were identified. Login to comment 187 ABCC2 p.Val417Ile This polymorphism causes a substitution of valine417 by isoleucine. Login to comment 215 ABCC5 p.Gly1249Ala Protein expression of MRP2, which has been established in a subset of the placental samples, did not differ in relation to the G1249A genotype [mean relative band intensity Ϯ S.D.; terms: 1249 GG, 0.77 Ϯ 0.28 (n ϭ 6), and terms: 1249 GA, 1.00 Ϯ 0.30 (n ϭ 7); Kruskal-Wallis test: ␹2 ϭ 1.479; df ϭ 1; p ϭ 0.224]. Login to comment 221 ABCC2 p.Val417Ile TABLE 3 C-24T G1249 (Val417Ile) and C3972T variants of the MRP2 gene in the population and in terms and preterms (preterm data in parentheses) Genotype Number Position Identified Frequency Expected Frequency Allele Allelic Frequency % C/C 37 -24 63.8 67.2 C 0.82 20 (17) 62.5 (65.4) 65.6 (68.9) 0.81 (0.83) C/T 21 -24 36.2 29.5 T 0.18 12 (9) 37.5 (34.6) 30.8 (28.2) 0.19 (0.17) T/T 0 -24 0 3.2 0 0 (0) 3.6 (2.9) G/G 37 1249 63.7 60.8 G 0.78 16 (21) 50.0 (80.8) 49.0 (74.0) 0.70 (0.86) G/A 16 1249 29.3 34.3 A 0.22 13 (3) 40.6 (11.5) 42.0 (22.4) 0.30 (0.13) A/A 5 1240 8.6 4.8 3 (2) 9.4 (7.7) 9.0 (1.8) C/C 22 3972 39.7 37.4 C 0.62 11 (11) 34.4 (46.1) 35.2 (40.3) 0.59 (0.65) C/T 27 3972 46.6 47.6 T 0.38 16 (11) 50 (42.3) 48.4 (45.5) 0.41 (0.35) T/T 9 3972 15.5 15.0 5 (4) 15.6 (15.4) 16.8 (12.2) 2) versus preterms: 1249 AA, 0.47 (n ϭ 2); Kruskal-Wallis test: ␹2 ϭ 1.895; df ϭ 2; p ϭ 0.428]. Login to comment 288 ABCB1 p.Gly1249Ala In the present paper, we studied the effect of three single nucleotide polymorphisms on the mRNA expression in human placenta, namely, the C-24T, G1249A, and C3972T. Login to comment 293 ABCB1 p.Gly1249Ala We found, however, a significant influence of the G1249A missense mutation on mRNA level in the present study. Login to comment