ABCMdb

PMID: 15613469

Bancila V, Cens T, Monnier D, Chanson F, Faure C, Dunant Y, Bloc A
Two SUR1-specific histidine residues mandatory for zinc-induced activation of the rat KATP channel.
J Biol Chem. 2005 Mar 11;280(10):8793-9. Epub 2004 Dec 21., [PubMed]

Sentences

No. Mutations Sentence Comment

6 ABCC8 p.His326Ala Of them, two histidines (His-326 and His-332) were found to be critical for the activation of KATP by zinc, as confirmed by the double mutation H326A/H332A. Login to comment 53 ABCC8 p.His326Ala ABCC8 p.His326Ala The double mutation H326A/H332A was done on the single cDNA mutant histidine 332 by using mutagenic primers (F/326 and R/326) introducing the H326A mutation. Login to comment 138 ABCC8 p.His160Ala ABCC8 p.His11Ala Mutations H11A, H160A, and H1273A had no effect, as zinc enhanced the KATP current in these clones to the same extent as in the wild type Kir6.2/SUR1 channels. Login to comment 139 ABCC8 p.His326Ala ABCC8 p.His326Ala In contrast, both mutations H326A and H332A, and even more so the double mutation H326A/H332A, converted the activation into inhibition. Login to comment 174 ABCC8 p.His326Ala ABCC8 p.His326Ala Mutations H326A and H327A and the double mutation H326A/H327A suppressed zinc activation of KATP. Login to comment 175 ABCC8 p.His160Ala ABCC8 p.His11Ala Mutations H11A, H160A, and H1273A did not affect the zinc activation of KATP current, whose amplitude remained equal to that of the wild type rat SUR1/Kir6.2 clone. Login to comment