ABCMdb

PMID: 15358601

Le Goff W, Peng DQ, Settle M, Brubaker G, Morton RE, Smith JD
Cyclosporin A traps ABCA1 at the plasma membrane and inhibits ABCA1-mediated lipid efflux to apolipoprotein A-I.
Arterioscler Thromb Vasc Biol. 2004 Nov;24(11):2155-61. Epub 2004 Sep 9., [PubMed]

Sentences

No. Mutations Sentence Comment

44 ABCA1 p.Val93Cys ApoAI Uptake, Degradation, and Resecretion Assay Radiolabeling of 140 òe;g of human ApoAI (specific activity 3620 cpm/ng) and assay of [125 I]apoAI uptake, degradation, and resecretion were performed as previously described.21 Uptake of Alexa568-labeled apoAI, processing, fixation, and DAPI counterstaining were as previously described,19 with the following modifications: an N-terminal His tagged ApoAI V93C substitution was created and purified by standard methods22 and labeled with Alexa 568 Maleimide (Molecular Probes). Login to comment 50 ABCA1 p.Val93Cys ApoAI Uptake, Degradation, and Resecretion Assay Radiolabeling of 140 ␮g of human ApoAI (specific activity 3620 cpm/ng) and assay of [125 I]apoAI uptake, degradation, and resecretion were performed as previously described.21 Uptake of Alexa568-labeled apoAI, processing, fixation, and DAPI counterstaining were as previously described,19 with the following modifications: an N-terminal His tagged ApoAI V93C substitution was created and purified by standard methods22 and labeled with Alexa 568 Maleimide (Molecular Probes). Login to comment