ABCMdb

PMID: 15336656

Virella-Lowell I, Herlihy JD, Liu B, Lopez C, Cruz P, Muller C, Baker HV, Flotte TR
Effects of CFTR, interleukin-10, and Pseudomonas aeruginosa on gene expression profiles in a CF bronchial epithelial cell Line.
Mol Ther. 2004 Sep;10(3):562-73., [PubMed]

Sentences

No. Mutations Sentence Comment

38 ABCC7 p.Trp1282* The cell lines used were IB3-1 (CFTR genotypeDF508/W1282X) and cells derived from IB3-1 that were either stably transduced to achieve low-level expression of full-length wild-type CFTR (S9) or transiently transfected with a high-expressing IL-10 construct. Login to comment 42 ABCC7 p.Trp1282* ABCC7 p.Trp1282* RESULTS General Oservations about Similarity of Gene Expression Profiles among Infected and Uninfected Cell Lines To characterize primary and secondary effects of CFTR mutation on gene expression, we extracted mRNA from cultures of the CF bronchial epithelial cell line IB3-1 (DF508/W1282X), the CFTR-corrected S9 line (DF508/ W1282X + wild-type CFTR), and IB3-1 cells with vector-mediated IL-10 expression. Login to comment