ABCMdb

PMID: 15331430

Chan DC, Watts GF, Barrett PH, Whitfield AJ, van Bockxmeer FM
ATP-binding cassette transporter G8 gene as a determinant of apolipoprotein B-100 kinetics in overweight men.
Arterioscler Thromb Vasc Biol. 2004 Nov;24(11):2188-91. Epub 2004 Aug 26., [PubMed]

Sentences

No. Mutations Sentence Comment

8 ABCG8 p.Asp19His No association was found with ABCG8 D19H. Login to comment 10 ABCG8 p.Asp19His No association was found with ABCG8 D19H. Login to comment 35 ABCG8 p.Asp19His ABCG8 p.Asp19His ABCG8 p.Asp19His ABCG8 p.Thr400Lys ABCG8 p.Thr400Lys ABCG8 p.Thr400Lys Plasma lathosterol, sitosterol, and campesterol concentrations were measured by gas-liquid chromatography and expressed in mmol/Lϫ102 per mol/L cholesterol.13,14 ABCG8 (T400K, D19H) and ApoE Genotyping ABCG8 (exon 1 D19H, exon 8 T400K) genotypes were determined by polymerase chain reaction amplification using as forward primer 5Ј AGG AAA CAG AGT GAA GAC ACT GG 3Ј and as reverse primer 5Ј AGA AAG GTT TGA TTT CTC CTA CCC 3Ј (T400K); and for D19H forward primer 5Ј ACA CCT GTG TGG AAA GGT AAG GT 3Ј and reverse primer 5Ј GCG GGT trichloroacetic acid GTA ATA AAA TGA CAG 3Ј as described by Hubacek et al.10 ApoE genotype was determined as described by Hixson and Vernier.15 Statistical Analysis All analyses were performed using SPSS 10.1 (SPSS). Login to comment 38 ABCG8 p.Asp19His ABCG8 p.Asp19His ABCG8 p.Asp19His ABCG8 p.Thr400Lys ABCG8 p.Thr400Lys ABCG8 p.Thr400Lys Plasma lathosterol, sitosterol, and campesterol concentrations were measured by gas-liquid chromatography and expressed in mmol/Lϫ102 per mol/L cholesterol.13,14 ABCG8 (T400K, D19H) and ApoE Genotyping ABCG8 (exon 1 D19H, exon 8 T400K) genotypes were determined by polymerase chain reaction amplification using as forward primer 5Ј AGG AAA CAG AGT GAA GAC ACT GG 3Ј and as reverse primer 5Ј AGA AAG GTT TGA TTT CTC CTA CCC 3Ј (T400K); and for D19H forward primer 5Ј ACA CCT GTG TGG AAA GGT AAG GT 3Ј and reverse primer 5Ј GCG GGT trichloroacetic acid GTA ATA AAA TGA CAG 3Ј as described by Hubacek et al.10 ApoE genotype was determined as described by Hixson and Vernier.15 Statistical Analysis All analyses were performed using SPSS 10.1 (SPSS). Login to comment 45 ABCG8 p.Asp19His ABCG8 p.Thr400Lys Allele frequencies of T400K were 0.86 (wild-type) and 0.14 (variant) and D19H were 0.92 (wild-type) and 0.08 (variant). Login to comment 46 ABCG8 p.Asp19His ABCG8 p.Thr400Lys Significant linkage disequilibrium was not found between T400K and D19H (DЈϭ0.44; Pϭ0.32). Login to comment 48 ABCG8 p.Asp19His ABCG8 p.Asp19His ABCG8 p.Thr400Lys Allele frequencies of T400K were 0.86 (wild-type) and 0.14 (variant) and D19H were 0.92 (wild-type) and 0.08 (variant). Login to comment 49 ABCG8 p.Asp19His ABCG8 p.Thr400Lys Significant linkage disequilibrium was not found between T400K and D19H (DЈϭ0.44; Pϭ0.32). Login to comment 51 ABCG8 p.Asp19His No significant influence of the ABCG8 D19H polymorphism on any lipid or anthropometric parameter was found. Login to comment 64 ABCG8 p.Thr400Lys Discussion This is the first study to demonstrate that ABCG8 (T400K) genotype may determine the metabolism of apoB in overweight/obese subjects. Our major finding was that compared with TT, ABCG8 TK individuals had lower VLDL-apoB PR and higher LDL production and FCRs. Login to comment 67 ABCG8 p.Thr400Lys Discussion This is the first study to demonstrate that ABCG8 (T400K) genotype may determine the metabolism of apoB in overweight/obese subjects. Our major finding was that compared with TT, ABCG8 TK individuals had lower VLDL-apoB PR and higher LDL production and FCRs. Login to comment