ABCMdb

PMID: 15304546

Sugita M, Kongo H, Shiba Y
Molecular dissection of the butyrate action revealed the involvement of mitogen-activated protein kinase in cystic fibrosis transmembrane conductance regulator biogenesis.
Mol Pharmacol. 2004 Nov;66(5):1248-59. Epub 2004 Aug 10., [PubMed]

Sentences

No. Mutations Sentence Comment

39 ABCC7 p.Ser660Ala ABCC7 p.Ser660Ala To construct the pEGFP-⌬R-CFTR mammalian expression vector, an AflII and SacI fragment from pTM1-CFTR⌬R-S660A containing the C-terminal region of CFTR was swapped with the wild-type cassette present in pEGFP-C1-CFTR. Login to comment 104 ABCC7 p.Ser660Ala In contrast, the B form of the deletion mutant GFP-⌬R-CFTR, which lacks much of the R domain and replaces Ser660 with alanine (Rich et al., 1993), was detected as an approximately 15-kDa increase in electrophoresis mobility (ϳ158 kDa) (Fig. 1B). Login to comment 215 ABCC7 p.Ser222Ala In the present study, LA- SDSE-MAPKK, in which Leu33 , Leu37 , Ser218 , and Ser222 were replaced by Ala, Ala, Asp, and Glu, respectively, was used as the constitutively active mutant (Fukuda et al., 1997). Login to comment 234 ABCC7 p.Ser222Ala We also checked the effect of cotransfection of the dominant-negative construct SASA-MAPKK, in which Ser218 and Ser222 were replaced by Ala (Gotoh et al., 1999); however, the cellular levels of active ERK/MAPKK were significantly reduced by CFTR transfection (Fig. 7A). Login to comment 303 ABCC7 p.Ser660Ala Acknowledgments We thank Dr. K. Foskett for providing pEGFP-CFTR, Dr. M. Welsh for CFTR⌬R-S660A, and Dr. E. Nishida for LA-SDSE-MAPKK and SASA-MAPKK. Login to comment