ABCMdb

PMID: 15229107

Chen Y, Altenberg GA, Reuss L
Mechanism of activation of Xenopus CFTR by stimulation of PKC.
Am J Physiol Cell Physiol. 2004 Nov;287(5):C1256-63. Epub 2004 Jun 30., [PubMed]

Sentences

No. Mutations Sentence Comment

33 ABCC7 p.Arg334Cys ABCC7 p.Arg334Cys XCFTR R334C was generated by substitution of Arg334 with Cys using the QuickChange multisite-directed mutagenesis kit (Stratagene, La Jolla, CA). Login to comment 126 ABCC7 p.Arg334Cys Oocytes expressing XCFTR-R334C and wild-type XCFTR had similar responses to PMA. Login to comment 146 ABCC7 p.Arg334Cys To test whether PMA stimulation causes insertion of new XCFTR channels, oocytes expressing the XCFTR-R334C mutant were first exposed to MTSET for 10-20 s, then the MTSET was thoroughly washed out and the oocytes were stimulated with PMA. Login to comment 163 ABCC7 p.Arg334Cys Effects of [2-(trimethylammonium)ethyl] methanethiosulfonate bromide (MTSET) on Gm of oocytes expressing XCFTR-R334C. Login to comment 211 ABCC7 p.Thr665Ala A nonspecific stimulatory effect of high PMA concentration can be ruled out because the same concentration of 4␣-PMA, an inactive form of PMA, had no effect, and the mutation T665A reduced the activation of XCFTR by PMA by ϳ75% (6). Login to comment