ABCMdb

PMID: 15191910

Lim M, McKenzie K, Floyd AD, Kwon E, Zeitlin PL
Modulation of deltaF508 cystic fibrosis transmembrane regulator trafficking and function with 4-phenylbutyrate and flavonoids.
Am J Respir Cell Mol Biol. 2004 Sep;31(3):351-7. Epub 2004 Jun 10., [PubMed]

Sentences

No. Mutations Sentence Comment

4 ABCC7 p.Trp1282* We sought to determine (i) the effect of apigenin, genistein, kaempferol, and quercetin on CFTR processing in IB3-1 cells (F508/ W1282X) and (ii) whether sequential treatment with 4-phenylbutyrate (4-PBA) to increase CFTR processing and flavonoid to directly stimulate CFTR would increase Cl-conductance. Login to comment 33 ABCC7 p.Trp1282* Materials and Methods Cell Culture IB3-1 (⌬F508/W1282X) bronchial epithelial cells (20) were cultured on uncoated T-75 flasks in LHC-8 supplemented with glutamine (gentamicin-free formulation; Biofluids, Rockville, MD), 5% bovine serum albumin (BSA) (Biofluids), penicillin-streptomycin (Gibco, BRL, Gaithersburg, MD), 1% fungizone (Biofluids), and 1% tobramycin (Eli Lily, Indianapolis, IN). Login to comment 77 ABCC7 p.Trp1282* The IB3-1 cell line contains one ⌬F508 allele and one W1282X allele. Login to comment