ABCMdb

PMID: 15155210

Chau AS, Mendrick CA, Sabatelli FJ, Loebenberg D, McNicholas PM
Application of real-time quantitative PCR to molecular analysis of Candida albicans strains exhibiting reduced susceptibility to azoles.
Antimicrob Agents Chemother. 2004 Jun;48(6):2124-31., [PubMed]

Sentences

No. Mutations Sentence Comment

116 ABCB1 p.Val437Ile ABCB1 p.Val437Ile MIC and RT-PCR data for SPRI azole-susceptible C. albicans isolates Isolate MICa (␮g/mL) ⌬CT Substitution(s) in Erg11p POS ITZ FLZ VOR ACT1 PMA1 ERG11 MDR1 CDR1 CDR2 C34 0.016 0.06 0.25 Ͻ0.004 8.1 7.1 10.3 11.1 11.3 13.5 None C40 0.016 0.06 0.25 Ͻ0.004 7.2 6.2 10.6 11.9 9.6 14.1 D116E, K128Tb C43 Ͻ0.03 0.06 0.25 0.03 7.4 6.3 10.3 15.1 10.2 14.4 NDc C60 0.016 0.06 Ͻ0.12 Ͻ0.004 7.6 6.3 8.6 10.9 11.8 15.9 None C72 0.03 0.06 0.06 Ͻ0.008 7.9 6.7 8.7 12.0 10.4 12.5 D116E C84 0.008 0.06 Ͻ0.12 Ͻ0.004 7.3 6.4 9.3 14.4 10.2 13.8 D116E C128 0.008 0.03 Ͻ0.12 Ͻ0.004 7.5 6.1 11.1 14.4 10.5 14.6 D116E, K128T,b D496 C132 0.016 0.06 0.25 Ͻ0.004 7.5 5.8 11.3 13.2 10.0 14.5 D116E, K128Tb C294 0.008 0.016 0.25 Ͻ0.008 8.9 8.3 10.9 12.8 12.3 16.0 None C392 Ͻ0.004 0.03 0.5 Ͻ0.008 7.9 6.7 11.1 14.0 11.2 14.6 None C393 Ͻ0.004 0.016 0.25 Ͻ0.008 7.8 6.7 11.3 13.0 10.7 14.7 ND C394 Ͻ0.004 0.016 0.25 Ͻ0.008 7.9 6.3 10.9 16.6 10.6 13.9 ND C395 Ͻ0.004 0.016 0.25 Ͻ0.008 7.9 6.8 11.5 12.4 10.7 15.6 V437I C548 Ͻ0.004 Ͻ0.008 Ͻ0.125 Ͻ0.008 7.6 6.3 10.0 14.2 12.3 14.4 V437I Avg ⌬CT 7.8 6.6 10.4 13.6 10.8 14.5 SD 0.4 0.6 0.9 2 0.8 0.9 3-SD range 6.6-9.0 4.8-8.4 7.7-13.1 7.6-19.6 8.4-13.2 11.8-17.2 a POS, posaconazole; ITZ, itraconazole; FLZ, fluconazole; VOR, voriconazole. Login to comment 126 ABCB1 p.Arg467Ile ABCB1 p.Arg467Ile MICa (␮g/ml) ⌬CT b Substitution(s) in Erg11p POS ITZ FLZ VOR ACT1 PMA1 ERG11 MDR1 CDR1 CDR2 1 C482 0.25 0.5 16 0.5 7.9 7.5 10.4 13.7 9.2 9.4 A107T C486 0.25 1 16 0.25 8.0 8.1 9.8 12.3 11.2 10.1 A107T 2 C491 0.12 0.25 2 0.03 7.4 7.2 8.9 12.9 10.1 10.0 D116E C504 0.25 0.25 16 0.25 7.3 6.2 7.9 15.5 8.2 7.7 F449S 3 C498 0.008 0.06 1 0.008 7.4 5.4 9.6 12.6 8.2 10.8 F145L, E266D, V488I C502 0.008 0.06 1 0.008 7.2 5.9 7.4 11.7 10.6 10.8 F145L, E266D, V488I C513 0.25 0.25 16 0.12 6.7 6.4 7.5 10.6 7.6 5.8 F145L, E266D, V488I 4 C527 0.12 0.5 256 4 7.5 6.6 9.1 12.6 8.9 8.6 Y132H, G450E C528 0.12 0.5 256 4 7.3 5.9 9.0 11.7 7.7 7.7 Y132H, G450E C530 0.12 0.25 256 0.5 7.0 6.3 8.5 12.0 8.8 9.9 G450E, G464S C534 0.12 0.5 256 4 7.3 6.4 8.1 11.0 8.3 8.8 Y132H, G450E C546 0.12 0.25 128 2 8.1 7.5 9.6 11.9 9.4 9.4 Y132H, G450E C536 0.25 0.5 256 4 8.2 7.7 9.8 11.7 9.2 9.4 Y132H, G450E C572 0.25 0.25 Ͼ256 8 8.0 7.4 9.5 12.1 9.5 9.2 Y132H, G450E 5 C481 0.25 0.5 32 0.5 7.9 7.2 9.9 15.7 9.4 9.0 F128T, F145L C483 0.25 0.5 8 0.12 8.2 7.3 11.0 14.8 10.0 9.5 F145L 6 C535 0.5 2 256 8 7.7 7.1 9.8 9.0 9.6 10.9 Y132H, G448V C539 0.25 2 128 8 7.2 6.8 11.0 8.3 6.6 7.1 Y132H, G448V 7 C516 0.12 0.25 32 0.5 7.3 7.1 9.8 14.0 9.7 9.7 K128T, Y132F, F145L 8 C470 0.5 0.5 32 0.25 7.3 7.0 9.0 13.5 8.7 8.5 S405F C478 0.5 0.5 64 0.5 7.6 6.9 8.6 12.6 9.1 8.5 S405F 9 C490 0.5 1 32 0.25 8.3 7.8 10.6 9.4 9.0 9.2 V452A C497 0.5 2 64 0.5 8.4 7.7 9.1 12.5 10.0 9.3 K128T, V452A 10 C526 0.5 1 32 0.5 7.8 7.1 9.6 13.6 9.2 9.2 V509M C533 0.5 0.5 32 1 7.9 7.4 9.8 13.6 9.3 9.9 V509M 11 C594 0.5 2 128 16 7.2 6.5 9.0 11.3 8.2 7.5 Y132H, S405F C600 1 4 Ͼ256 16 7.7 7.1 8.5 12.3 9.4 8.2 Y132H, S405F 12 C577 0.06 0.06 128 0.5 6.5 5.0 9.0 5.3 8.1 12.9 G464S C583 0.25 0.25 256 1 6.8 5.5 9.4 5.7 6.9 10.7 G464S C587 0.5 0.5 256 4 7.7 7.3 9.2 8.2 10.2 9.6 G464S 13 C480 0.03 0.12 128 8 8.3 7.4 9.3 9.2 12.2 15.1 K128T, G464S, R467I 14 C438 0.5 0.5 128 2 6.6 6.0 7.9 13.3 7.1 7.5 Y257H, G464S C439 1 4 Ͼ256 16 7.1 6.2 7.6 13.5 7.7 7.5 Y257H, G464S, G307S C440 4 8 Ͼ256 Ͼ16 7.3 6.4 8.0 13.5 7.9 7.7 Y257H, G464S, G307S, A61V 15 C444 0.5 0.5 256 0.5 7.0 5.8 7.4 13.2 7.8 7.8 K143R 16 C489 0.25 0.25 128 1 7.2 6.1 8.8 12.8 10.1 9.5 Y132H, Y257H, E266D 17 C477 1 0.25 256 16 7.6 6.1 9.3 7.3 10.5 15.3 K128T, G464S, R467I 18 C507 1 2 64 8 6.1 7.2 8.6 10.5 8.3 8.4 Y132H, H283R, G464S a See Table 2, footnote a. b Values that fall outside the 3-SD range measured in the azole-susceptible isolates (see text for details) are shown in boldface italic. Login to comment 186 ABCB1 p.Arg467Ile ABCB1 p.Arg467Ile MIC (␮g/ml) ⌬CT Substitution(s) in Erg11p POS ITZ FLZ VOR ACT1 PMA1 ERG11 MDR1 CDR1 CDR2 4 C527 0.12 0.5 256 4 7.5 6.6 9.1 12.6 8.9 8.6 Y132H, G450E C528 0.12 0.5 256 4 7.3 5.9 9.0 11.7 7.7 7.7 Y132H, F155L, G450E C534 0.12 0.5 256 4 7.3 6.4 8.1 11.0 8.3 8.8 Y132H, G450E C546 0.12 0.25 128 2 8.1 7.5 9.6 11.9 9.4 9.4 Y132H, G450E C536 0.25 0.5 256 4 8.2 7.7 9.8 11.7 9.2 9.4 Y132H, G450E C572 0.25 0.25 Ͼ256 8 8.0 7.4 9.5 12.1 9.5 9.2 Y132H, G450E 6 C535 0.5 2 256 8 7.7 7.1 9.8 9.0 9.6 10.9 Y132H, G448V C539 0.25 2 128 8 7.2 6.8 11.0 8.3 6.6 7.1 Y132H, G448V 11 C594 0.5 2 128 16 7.2 6.5 9.0 11.3 8.2 7.5 Y132H, S405F C600 1 4 Ͼ256 16 7.7 7.1 8.5 12.3 9.4 8.2 Y132H, S405F 12 C587 0.5 0.5 256 4 7.7 7.3 9.2 8.2 10.2 9.6 G464S 13 C480 0.03 0.12 128 8 8.3 7.4 9.3 9.2 12.2 15.1 K128T, G464S, R467I 14 C438 0.5 0.5 128 2 6.6 6.0 7.9 13.3 7.1 7.5 Y257H, G464S C439 1 4 Ͼ256 16 7.1 6.2 7.6 13.5 7.7 7.5 Y257H, G464S, G307S C440 4 8 Ͼ256 Ͼ16 7.3 6.4 8.0 13.5 7.9 7.7 Y257H, G464S, G307S, A61V 17 C477 1 0.25 256 16 7.6 6.1 9.3 7.3 10.5 15.3 K128T, G464S, R467I 18 C507 1 2 64 8 6.1 7.2 8.6 10.5 8.3 8.4 Y132H, H283R, G464S a See Table 2, footnote a, and Table 3, footnote b. TABLE 5. Login to comment 187 ABCB1 p.Arg467Ile Changes in azole susceptibility resulting from expressing C. albicans ERG11 alleles in S. cerevisiae Original C. albicans isolate Substitution(s) in expressed C. albicans Erg11p MICa (␮g/ml) POS ITZ FLZ VOR None None (vector alone) 0.06 0.125 4 0.016 C43 None (wild type) 0.06 0.5 16 0.125 C441 K143R 0.25 1 Ͼ256 1 C587 G464S 0.25 1 Ͼ256 1 C477 K128T, G464S, R467I 0.125 0.5 32 0.25 C530 G450E, G464S 1 1 128 1 C600 Y132H, S405F 0.5 Ͼ8 Ͼ256 16 C535 Y132H, G448V 0.25 1 Ͼ256 0.03 C572 Y132H, G450E 0.5 2 Ͼ256 8 C438 Y257H, G464S 0.5 1 128 4 C439 Y257H, G464S, G307S 1 1 Ͼ256 8 C440 Y257H, G464S, G307S, A61V 4 8 Ͼ256 16 NAb A61V 0.25 0.5 16 0.125 a See Table 2, footnote a. b NA, not applicable; mutation was engineered into a wild-type ERG11 gene. Login to comment