ABCMdb

PMID: 15131592

Tainton KM, Smyth MJ, Jackson JT, Tanner JE, Cerruti L, Jane SM, Darcy PK, Johnstone RW
Mutational analysis of P-glycoprotein: suppression of caspase activation in the absence of ATP-dependent drug efflux.
Cell Death Differ. 2004 Sep;11(9):1028-37., [PubMed]

Sentences

No. Mutations Sentence Comment

23 ABCB1 p.Lys1076Met As shown in Figure 1 (top panel), cells transduced with MSCV alone (CEM-GFP), MSCV-MDR1 (CEM-P-gpWT ) and two clones transduced with MSCV- MDR1K433M/K1076M (CEM-P-gpMM-3 and CEM-P-gpMM-32 ) were obtained and expressed equivalent levels of intracellular GFP. Login to comment 26 ABCB1 p.Lys1076Met ABCB1 p.Lys433Met To confirm that the desired form of P-gp was expressed, the regions encoding K433M and K1076M substitutions were amplified by PCR using cDNA obtained from CEM-P-gpWT , CEM-P-gpMM-3 and CEM-P-gpMM-32 cells and sequenced (data not shown). Login to comment 164 ABCB1 p.Lys1076Met Production of retrovirally transduced cell lines Production of MSCV-MDR1 (wild type) and MSCV-MDR1 (K433 M, K1076M) has been previously described.41 These constructs or MSCV alone were transfected into 293T cells with PEQ and RD114 helper viruses by CaPO4 À precipitation. Login to comment 170 ABCB1 p.Lys433Met Primers for K433M were: (forward) TTGAAGGGTCTGAACCTG and (reverse) TGGTTGCAGGCCTCCATT- TA. Login to comment 171 ABCB1 p.Lys1076Met Primers for K1076M were: (forward) ATCCCAGTGCTTCAGGGA and (reverse) CCTTATTCCAAGCGGCTT. Login to comment