ABCMdb

PMID: 15047694

Yan W, Samaha FF, Ramkumar M, Kleyman TR, Rubenstein RC
Cystic fibrosis transmembrane conductance regulator differentially regulates human and mouse epithelial sodium channels in Xenopus oocytes.
J Biol Chem. 2004 May 28;279(22):23183-92. Epub 2004 Mar 26., 2004-05-28 [PubMed]

Sentences

No. Mutations Sentence Comment

35 ABCC7 p.Thr663Ala We also tested the hypothesis that a naturally occurring polymorphism in the C terminus of ␣ hENaC, substitution of Ala at residue 663 for Thr (T663A), which we have recently shown to decrease the functional and surface expression of hENaC in oocytes,2 would influence regulatory interactions between CFTR and hENaC. Login to comment 79 ABCC7 p.Thr663Ala Co-expression of CFTR and hENaC-We next assessed regulatory interactions between CFTR and hENaC as well as the potential influence of the T663A functional polymorphism of ␣ hENaC described recently by our group2 on these interactions. Login to comment 120 ABCC7 p.Thr663Ala We recently observed that the C-terminal 20 residues (residues 650-669) of ␣ hENaC, when expressed at the C terminus of a murine/human ␣ ENaC chimera in place of the 21 C-terminal amino acids of ␣ mENaC (residues 679-699), were sufficient to confer functionality of the T663A polymorphism of ␣ hENaC.2 Given that mENaC (Fig. 1 and Ref. Login to comment 134 ABCC7 p.Thr663Ala As our data suggest differences between the interregulation of wild type CFTR and hENaC versus wild type CFTR and mENaC, we sought to characterize regulatory interactions between ⌬F508-CFTR and hENaC as well as the potential influence of the T663A functional polymorphism on these interactions (Fig. 7). Login to comment