ABCMdb

PMID: 15039072

Roosbeek S, Caster H, Liu QZ, Berne PF, Duverger N, Christiaens B, Vandekerckhove J, Peelman F, Labeur C, Rosseneu M
Expression and activity of the nucleotide-binding domains of the human ABCA1 transporter.
Protein Expr Purif. 2004 May;35(1):102-10., [PubMed]

Sentences

No. Mutations Sentence Comment

85 ABCA1 p.Gly2074Asn ABCA1 p.Gly1062Gln ABCA1 p.Gly1062Asn ABCA1 p.Thr2073Asn ABCA1 p.Ala1061Asn ABCA1 p.Asp1064Ala ABCA1 p.Asp1064Ala ABCA1 p.Asp2076Ala ABCA1 p.Asp2076Ala To test if these residues are important for ATP binding and ATPase activity, we engineered and expressed the following mutations: A1061N, G1062N, D1064A, G1062Q + D1064A in NBD1, and T2073N, G2074N, D2076A, G2074Q+ D2076A in NBD2. Login to comment 119 ABCA1 p.Gly1062Gln ABCA1 p.Asp1064Ala ABCA1 p.Asp2076Ala ABCA1 p.Gly2074Gln Size determination of WT and mutant ABCA1 His-NBD proteins by Dynamic Light Scattering: (s) His-NBD1 WT; (m) His-NBD1 (G1062Q + D1064A); (j) His-NBD1 (T1242 + T1243); (Ã) His-NBD2 WT; and (Ã) His-NBD2 (G2074Q + D2076A). Login to comment 120 ABCA1 p.Gly1062Gln ABCA1 p.Asp1064Ala ABCA1 p.Asp2076Ala ABCA1 p.Gly2074Gln Size determination of WT and mutant ABCA1 His-NBD proteins by Dynamic Light Scattering: (s) His-NBD1 WT; (m) His-NBD1 (G1062Q + D1064A); (j) His-NBD1 (T1242 + T1243); () His-NBD2 WT; and () His-NBD2 (G2074Q + D2076A). Login to comment