PMID: 15000840

De Semir D, Aran JM
Misleading gene conversion frequencies due to a PCR artifact using small fragment homologous replacement.
Oligonucleotides. 2003;13(4):261-9., [PubMed]
Sentences
No. Mutations Sentence Comment
53 ABCC7 p.Trp1282*
X
ABCC7 p.Trp1282* 15000840:53:231
status: NEW
view ABCC7 p.Trp1282* details
 We wished to improve the conditions to obtain maximal correction of the CFTR mutation F508del in our cellular model, the adeno-SV40-transformed bronchial epithelial cell line IB3.1, isolated from a CF patient with genotype F508del/W1282X (Zeitlin et al., 1991). Login to comment 57 ABCC7 p.Trp1282*
X
ABCC7 p.Trp1282* 15000840:57:74
status: NEW
view ABCC7 p.Trp1282* details
 Similarly as performed with the detection of the oligonucleotide-mediated W1282X correction frequency in IB3.1 cells (De Semir et al., 2003), we optimized the analysis of the percentage of F508del mutation repair in a heterozygous background using exponential PCR and genotyping. Login to comment