ABCMdb

PMID: 14744818

Derand R, Montoni A, Bulteau-Pignoux L, Janet T, Moreau B, Muller JM, Becq F
Activation of VPAC1 receptors by VIP and PACAP-27 in human bronchial epithelial cells induces CFTR-dependent chloride secretion.
Br J Pharmacol. 2004 Feb;141(4):698-708. Epub 2004 Jan 26., [PubMed]

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25 ABCC7 p.Trp1282* IB3-1 (delF508/W1282X) were generously given by Dr P. Zeitlin (Zeitlin et al., 1991) and routinely cultured at 371C in 5% CO2 incubators in LHC-8 medium (Biofluids, Inc., Rockville, MO, U.S.A.) supplemented with 10% fetal bovine serum and 100 IU mlÀ1 penicillin/ streptomycin (Bulteau et al., 2000). Login to comment 174 ABCC7 p.Trp1282* Specificity of VIP and PACAP-27 towards CFTR In this part of the study, we used the human airway epithelial IB3-1 cells (Zeitlin et al., 1991) derived from a CF patient (delF508/W1282X) because CFTR is absent from the plasma membrane due to its abnormal trafficking (Zeitlin et al., 1991; Dormer et al., 2001). Login to comment