ABCMdb

PMID: 14701824

Haidar B, Denis M, Marcil M, Krimbou L, Genest J Jr
Apolipoprotein A-I activates cellular cAMP signaling through the ABCA1 transporter.
J Biol Chem. 2004 Mar 12;279(11):9963-9. Epub 2003 Dec 29., [PubMed]

Sentences

No. Mutations Sentence Comment

9 ABCA1 p.Cys1477Arg Naturally occurring mutations of ABCA1 associated with Tangier disease (C1477R, 2203X, and 2145X) severely reduced apoA-I-mediated cAMP production, ABCA1 phosphorylation, 125 I-apoA-I binding, and lipid efflux, without affecting forskolin-mediated cAMP elevation. Login to comment 153 ABCA1 p.Cys1477Arg The C1477R (TD-1) mutant showed decreased presence at the plasma membrane, relative to the normal transporter (50% or greater reduction); however, the 2154X (TD-3) mutant failed to reach the membrane. Login to comment 154 ABCA1 p.Cys1477Arg The C1477R (TD-1) mutant showed decreased presence at the plasma membrane, relative to the normal transporter (50% or greater reduction); however, the 2154X (TD-3) mutant failed to reach the membrane. Login to comment 198 ABCA1 p.Cys1477Arg On the other hand, we have documented previously (8) that 8-Br-cAMP and FRK did not induce ABCA1 phosphorylation in intact fibroblasts from TD-1 (C1477R) as compared with normal cells. Login to comment 200 ABCA1 p.Cys1477Arg On the other hand, we have documented previously (8) that 8-Br-cAMP and FRK did not induce ABCA1 phosphorylation in intact fibroblasts from TD-1 (C1477R) as compared with normal cells. Login to comment 206 ABCA1 p.Cys1477Arg A, fibroblasts from a normal control (CTR) and TD subjects (TD-1 (C1477R), TD-2 (2203X), and TD-3 (2145X)) (Table I) were stimulated, and ABCA1 was immunoprecipitated and incubated with [ॹ-32 P]ATP in the absence or presence of the PKA catalytic subunit (PKA-c) as described under "Experimental Procedures." Login to comment 208 ABCA1 p.Cys1477Arg A, fibroblasts from a normal control (CTR) and TD subjects (TD-1 (C1477R), TD-2 (2203X), and TD-3 (2145X)) (Table I) were stimulated, and ABCA1 was immunoprecipitated and incubated with [␥-32 P]ATP in the absence or presence of the PKA catalytic subunit (PKA-c) as described under "Experimental Procedures." Login to comment