ABCMdb

PMID: 14602047

Howell LD, Borchardt R, Kole J, Kaz AM, Randak C, Cohn JA
Protein kinase A regulates ATP hydrolysis and dimerization by a CFTR (cystic fibrosis transmembrane conductance regulator) domain.
Biochem J. 2004 Feb 15;378(Pt 1):151-9., 2004-02-15 [PubMed]

Sentences

No. Mutations Sentence Comment

84 ABCC7 p.Ser768Ala Similarly, the substitution of Ala for Ser-768 resulted in the loss of phosphopeptides 2, 3 and 8, indicating that these three peptides contain Ser-768 (Figure 1E). Login to comment 173 ABCC7 p.Gly551Asp First, kinetic studies showed that the G551D mutation changed the Km but not the Vmax of the NBD1-R-GST preparation [14]. Login to comment 175 ABCC7 p.Gly551Asp ABCC7 p.Gly551Asp Thus even though a contaminating ATPase could have led to differences in the total measured ATPase activity of the G551D versus wild-type fusion protein preparations (e.g. if the G551D mutation affected the tendency of contaminants to associate with NBD1-R-GST during its purification), these findings indicate that NBD1-R-GST is the protein responsible for the observed ATP binding and ATP hydrolysis properties of the preparation. Login to comment