ABCMdb

PMID: 14596908

Forestier C, Frangne N, Eggmann T, Klein M
Differential sensitivity of plant and yeast MRP (ABCC)-mediated organic anion transport processes towards sulfonylureas.
FEBS Lett. 2003 Nov 6;554(1-2):23-9., [PubMed]

Sentences

No. Mutations Sentence Comment

31 ABCC7 p.Glu217Gly ABCC7 p.Glu217Gly Abbreviations: ABC, ATP-binding cassette; BCECF, (2P,7P-bis-(2-carboxyethyl)-5-(and-6-)carboxy£uorescein; BmCl, monochlorobi- mane; CFTR, cystic 'brosis transmembrane conductance regulator; DNB-GS, 2,4-dinitrobenzene glutathione; E217G, L-estradiol 17-(L-D- glucuronide); GSH, reduced glutathione; GSSG, oxidized glutathione; GS-X, glutathione conjugate; KCO, potassium channel opener; LY-CH, lucifer yellow CH; MRP, multidrug resistance-associated protein; SUR, sulfonylurea receptor FEBS 27703 23-10-03 Cyaan Magenta Geel Zwart FEBS 27703 FEBS Letters 554 (2003) 23^29 trol other membrane proteins and more speci'cally ion channels [17]. Login to comment 69 ABCC7 p.Glu217Gly Protoplast preparations were incapable of transporting glutathione conjugates or E217G. Login to comment 92 ABCC7 p.Glu217Gly Results It has been demonstrated that isolated vacuoles or tonoplast vesicles from various plant sources take up GS-X as well as E217G using directly energized transport systems [6,10,33]. Login to comment 107 ABCC7 p.Glu217Gly Furthermore, cromakalim does not antagonize glucuronide transport inhibition by gli- Fig. 1. Dose-dependent inhibition of vacuolar E217G uptake into barley vacuoles by glibenclamide in the absence (circles) or presence (squares) of 0.2 mM DNB-GS. Login to comment 108 ABCC7 p.Glu217Gly Barley mesophyll vacuoles were incubated with 11.4 nM E217G in the presence of 4 mM MgCl2, 3 mM ATP and glibenclamide at the concentrations indicated. Login to comment 113 ABCC7 p.Glu217Gly ABCC7 p.Glu217Gly Table 1 E¡ects of sulfonylureas and KCOs on the ATP-dependent uptake of [3 H]E217G into barley vacuoles Condition Vacuolar transport of [3 H]E217G without DNB-GS +0.2 mM DNB-GS % of MgATP-stimulated value Control 100 832 þ 320 +150 WM glibenclamide (glib) 12.9 þ 12.7 36 þ 29 +0.1 mM tolbutamide 88.1 þ 7.4 638 þ 9 +1 mM tolbutamide 81.6 þ 5.2 599 þ 86 +10 WM cromakalim (crom) 90.2 þ 2.2 500 þ 15 +100 WM crom 63 þ 9.5 518 þ 13 +1 WM SR47063 87.9 þ 18.53 536 þ 17 +10 WM SR47063 35.6 þ 20.7 298 þ 61 +10 WM RP49356 79.2 þ 4.67 595 þ 11 +100 WM RP49356 63.1 þ 2.62 373 þ 29 +150 WM glib+100 WM crom 2.8 þ 3.9 49.4 þ 5 +15 WM glib+100 WM crom 64.1 þ 5.2 429 þ 56 Barley mesophyll vacuoles were incubated with 11.4 nM E217G in the presence of 4 mM MgCl2, 3 mM ATP, in the absence or presence of DNB-GS as a stimulating agent and diverse pharmacological compounds interfering with CFTR and SUR. Login to comment 119 ABCC7 p.Glu217Gly Vacuoles isolated from an Arabidopsis cell culture exhibited a directly energized transport systems for DNB-GS and E217G. Login to comment 121 ABCC7 p.Glu217Gly Thus, the glibenclamide sensitivity of directly energized transport systems for DNB-GS and E217G is not species-speci'c in plants. Login to comment 123 ABCC7 p.Glu217Gly ABCC7 p.Glu217Gly Glibenclamide selectively inhibits the vacuolar deposition of anionic £uorescent dyes in barley vacuoles Since glibenclamide was a very e/cient inhibitor of E217G but not DNB-GS transport, we investigated its action on the vacuolar accumulation of di¡erent negatively charged £uorescent probes used as substrates for ABC-type transport processes. Login to comment 126 ABCC7 p.Glu217Gly Vacuolar LY-CH uptake was stimulated by 0.2 mM DNB-GS up to about 920% of the control suggesting a similar interaction with GS-Xs as shown for E217G. Login to comment 127 ABCC7 p.Glu217Gly Again, glibenclamide strongly inhibited the DNB-GS-stimulated LY-CH uptake into barley vacuoles (from about 920 to about 5%; Fig. 2) suggesting a comparable pharmacology as shown for E217G. Login to comment 132 ABCC7 p.Glu217Gly In contrast to LY-CH and E217G, the vacuolar uptake of BCECF was not stimulated by di¡erent GS-X and was inhibited by oxidized glutathione (GSSG) (Table Table 3 BCECF uptake into barley vacuoles is mediated by an ABC-type transporter Treatment Uptake of BCECF % of MgATP-stimulated value 3ATP 0.1 þ 0.1 +MgATP (control) 100 +MgATP +1 mM vanadate 5.1 þ 7.3 +MgATP +0.1 WM ba'lomycin A1 103.9 þ 26.0 +MgATP +5 mM NH4Cl 129.4 þ 24.7 +MgATP +150 WM glibenclamide 0.0 þ 2.2 +MgATP +3 mM GSSG 55.1 þ 13.4 +MgATP +3 mM GSH 89.8 þ 15.4 +MgATP +0.2 mM DNB-GS 115.5 þ 6.2 +MgATP +0.2 mM Decyl-GS 93.8 þ 19.8 Isolated barley vacuoles were incubated with 1 mM MgSO4 (3ATP) or 3 mM ATP and 4 mM MgSO4 (+MgATP), 25 WM BCECF and further compounds as indicated. Login to comment 196 ABCC7 p.Glu217Gly This hypothesis is in accordance with the fact that the CFTR chloride channel is blocked by glibenclamide and taurocholate or E217G [42]. Login to comment 197 ABCC7 p.Glu217Gly This hypothesis is in accordance with the fact that the CFTR chloride channel is blocked by glibenclamide and taurocholate or E217G [42]. Login to comment 207 ABCC7 p.Glu217Gly Swanson et al. [34] suggested the presence of a vacuolar ABC-type transport system for BCECF in bar- Fig. 5. Dose-dependent glibenclamide inhibition of the ATP-stimulated uptake of E217G (closed circles) and DNB-GS (open circles) in yeast vacuoles. Login to comment 208 ABCC7 p.Glu217Gly ABCC7 p.Glu217Gly Yeast vacuoles isolated from the W303-A1 strain were incubated with 10 WM E217G or 15 WM DNB-GS in the presence of 5 mM MgATP. Login to comment 209 ABCC7 p.Glu217Gly Yeast vacuoles isolated from the W303-A1 strain were incubated with 10 WM E217G or 15 WM DNB-GS in the presence of 5 mM MgATP. Login to comment