ABCMdb

PMID: 12869555

Martinez LO, Agerholm-Larsen B, Wang N, Chen W, Tall AR
Phosphorylation of a pest sequence in ABCA1 promotes calpain degradation and is reversed by ApoA-I.
J Biol Chem. 2003 Sep 26;278(39):37368-74. Epub 2003 Jul 17., [PubMed]

Sentences

No. Mutations Sentence Comment

5 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala The ABCA1-T1286A/T1305A mutant was not degraded by calpain and was not further stabilized upon apoA-I treatment. Login to comment 6 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala The T1286A/T1305A mutant showed a 3.1-fold increase in cell surface expression and a 2.3-fold increase of apoAI-mediated cholesterol efflux compared with wild type ABCA1. Login to comment 30 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala ABCA1 p.Ser1302Ala ABCA1 p.Ser1302Ala ABCA1 p.Ser1296Ala ABCA1 p.Ser1296Ala mABCA1-FLAG mutation constructs were further called: MutAAAA for mutation to Ala on T1286A/S1296A/S1302A/T1305A, MutTAAT for mutation to Ala on S1296A/S1302A, and MutASSA for mutation to Ala on * This work was supported in part by National Institutes of Health Grant HL22682. Login to comment 31 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala ABCA1 p.Ser1302Ala ABCA1 p.Ser1302Ala ABCA1 p.Ser1296Ala ABCA1 p.Ser1296Ala mABCA1-FLAG mutation constructs were further called: MutAAAA for mutation to Ala on T1286A/S1296A/S1302A/T1305A, MutTAAT for mutation to Ala on S1296A/S1302A, and MutASSA for mutation to Ala on * This work was supported in part by National Institutes of Health Grant HL22682. Login to comment 38 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala 39, Issue of September 26, pp. 37368-37374, 2003 (c) 2003 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in U.S.A. This paper is available on line at http://www.jbc.org T1286A/T1305A. Login to comment 41 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala Printed in U.S.A. This paper is available on line at http://www.jbc.org T1286A/T1305A. Login to comment 118 ABCA1 p.Thr1305Ala ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala ABCA1 p.Thr1286Ala ABCA1 p.Ser1302Ala ABCA1 p.Ser1302Ala ABCA1 p.Ser1296Ala ABCA1 p.Ser1296Ala Mutation of Thr residues (1286 and 1305) decreases ABCA1 Phosphorylation. WT-mABCA1-FLAG, mABCA1delPEST-FLAG, mABCA1-FLAG mutated to Ala on T1286A/S1296A/S1302A/T1305A (MutAAAA), mutated to Ala on S1296A/S1302A (MutTAAT), or mutated to Ala on T1286A/T1305A (MutASSA), were transiently transfected in 6 wells of HEK-293 cells (2 òe;g of plasmid DNA per well). Login to comment 121 ABCA1 p.Thr1305Ala ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala ABCA1 p.Thr1286Ala ABCA1 p.Ser1302Ala ABCA1 p.Ser1302Ala ABCA1 p.Ser1296Ala ABCA1 p.Ser1296Ala Mutation of Thr residues (1286 and 1305) decreases ABCA1 Phosphorylation. WT-mABCA1-FLAG, mABCA1delPEST-FLAG, mABCA1-FLAG mutated to Ala on T1286A/S1296A/S1302A/T1305A (MutAAAA), mutated to Ala on S1296A/S1302A (MutTAAT), or mutated to Ala on T1286A/T1305A (MutASSA), were transiently transfected in 6 wells of HEK-293 cells (2 ␮g of plasmid DNA per well). Login to comment 147 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala ABCA1 mutated on Thr-1286 and Thr-1305 is not degraded by calpain and is not stabilized by apoA-I. WT-mABCA1-FLAG or mABCA1-FLAG mutated to Ala on T1286A/T1305A (MutTSSA) were transiently transfected into HEK293 cells. Login to comment 150 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala ABCA1 mutated on Thr-1286 and Thr-1305 is not degraded by calpain and is not stabilized by apoA-I. WT-mABCA1-FLAG or mABCA1-FLAG mutated to Ala on T1286A/T1305A (MutTSSA) were transiently transfected into HEK293 cells. Login to comment 162 ABCA1 p.Trp590Ser A mutant (ABCA1-W590S) showed normal levels of binding of apoA-I but decreased phospholipid efflux (22, 23). Login to comment 165 ABCA1 p.Trp590Ser A mutant (ABCA1-W590S) showed normal levels of binding of apoA-I but decreased phospholipid efflux (22, 23). Login to comment 186 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala Effect of apigenin (CK2 inhibitor) and H-89 (PKA inhibitor) on ABCA1 phosphorylation. WT-mABCA1-FLAG or mABCA1-FLAG mutated to Ala on T1286A/T1305A (MutASSA) were transiently transfected in 6 wells of HEK-293 cells (2 òe;g of plasmid DNA per well). Login to comment 189 ABCA1 p.Thr1305Ala ABCA1 p.Thr1286Ala Effect of apigenin (CK2 inhibitor) and H-89 (PKA inhibitor) on ABCA1 phosphorylation. WT-mABCA1-FLAG or mABCA1-FLAG mutated to Ala on T1286A/T1305A (MutASSA) were transiently transfected in 6 wells of HEK-293 cells (2 ␮g of plasmid DNA per well). Login to comment