PMID: 12676769

Zabner J, Karp P, Seiler M, Phillips SL, Mitchell CJ, Saavedra M, Welsh M, Klingelhutz AJ
Development of cystic fibrosis and noncystic fibrosis airway cell lines.
Am J Physiol Lung Cell Mol Physiol. 2003 May;284(5):L844-54. Epub 2003 Jan 10., [PubMed]
Sentences
No. Mutations Sentence Comment
140 ABCC7 p.Gly551Asp
X
ABCC7 p.Gly551Asp 12676769:140:269
status: NEW
view ABCC7 p.Gly551Asp details
ABCC7 p.Arg553*
X
ABCC7 p.Arg553* 12676769:140:232
status: NEW
view ABCC7 p.Arg553* details
Immortal normal and CF HAE cell lines Cell Line CF Genotype Rt, ⍀⅐cm2 NuLi-1 WT Ͼ450 NuLi-2 WT Ͻ50 CuFi-1 ⌬F508/⌬F508 Ͼ450 CuFi-2 ⌬F508/⌬F508 Ͻ50 CuFi-3 ⌬F508/R553X Ͼ450 CuFi-4 ⌬F508/G551D Ͼ450 Cells were transduced with hTERT and human papilloma virus type 16 E6/E7 retroviruses. Login to comment
190 ABCC7 p.Gly551Asp
X
ABCC7 p.Gly551Asp 12676769:190:277
status: NEW
view ABCC7 p.Gly551Asp details
ABCC7 p.Arg553*
X
ABCC7 p.Arg553* 12676769:190:245
status: NEW
view ABCC7 p.Arg553* details
Primary lung epithelial cells from CF lung transplant recipients were infected with hTERT and HPV-16 E6/E7 to generate four different immortalized CF cells: CuFi-1 (⌬508/⌬508), CuFi-2 (⌬508/⌬508), CuFi-3 (⌬508/R553X), and CuFi-4 (⌬508/G551D). Login to comment
241 ABCC7 p.Gly551Asp
X
ABCC7 p.Gly551Asp 12676769:241:191
status: NEW
view ABCC7 p.Gly551Asp details
ABCC7 p.Arg553*
X
ABCC7 p.Arg553* 12676769:241:156
status: NEW
view ABCC7 p.Arg553* details
Differentiated primary cultures of airway epithelial cells from 3 different immortalized CF cells: CuFi-1 (⌬508/⌬508, A), CuFi-3 (⌬508/R553X, B), and CuFi-4 (⌬508/G551D, C) at passage 18 were individually thawed and seeded onto semipermeable filters and compared with their primary source. Login to comment
258 ABCC7 p.Gly551Asp
X
ABCC7 p.Gly551Asp 12676769:258:71
status: NEW
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Several groups have screened compounds that may affect ⌬F508 or G551D CFTR-expressing cells to restore Cl-channel function (3, 8, 22, 34, 36, 47). Login to comment
267 ABCC7 p.Arg553*
X
ABCC7 p.Arg553* 12676769:267:26
status: NEW
view ABCC7 p.Arg553* details
CuFi-3 cells that express R553X (2, 5, 16, 47) could be an excellent candidate for screening those drugs. Login to comment