ABCMdb

PMID: 12588899

Chappe V, Hinkson DA, Zhu T, Chang XB, Riordan JR, Hanrahan JW
Phosphorylation of protein kinase C sites in NBD1 and the R domain control CFTR channel activation by PKA.
J Physiol. 2003 Apr 1;548(Pt 1):39-52. Epub 2003 Feb 14., 2003-04-01 [PubMed]

Sentences

No. Mutations Sentence Comment

14 ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala ABCC7 p.Thr682Ala ABCC7 p.Thr791Ala ABCC7 p.Ser809Ala ABCC7 p.Thr604Ala ABCC7 p.Ser707Ala ABCC7 p.Thr582Ala ABCC7 p.Ser641Ala To examine if these effects are mediated by direct PKC phosphorylation of CFTR, a mutant was constructed in which serines or threonines at nine PKC consensus sequences on CFTR were replaced by alanines (i.e. the '9CA` mutant T582A/T604A/S641A/T682A/S686A/S707A/S790A/T791A/S809A). Login to comment 145 ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala ABCC7 p.Thr682Ala ABCC7 p.Thr791Ala ABCC7 p.Ser809Ala ABCC7 p.Thr604Ala ABCC7 p.Ser707Ala ABCC7 p.Thr582Ala ABCC7 p.Ser641Ala To distinguish these possible mechanisms we constructed a mutant (9CA) in which all PKC consensus sequences between the Walker B motif of NBD1 and second transmembrane domain (TMD2; i.e. the seventh membrane-spanning segment; T582A, T604A, S641A, T682A, S686A, S707A, S790A, T791A and S809A) were eliminated (Fig. 4A and B). Login to comment