PMID: 12471126

Castelli FA, Buhot C, Sanson A, Zarour H, Pouvelle-Moratille S, Nonn C, Gahery-Segard H, Guillet JG, Menez A, Georges B, Maillere B
HLA-DP4, the most frequent HLA II molecule, defines a new supertype of peptide-binding specificity.
J Immunol. 2002 Dec 15;169(12):6928-34., [PubMed]
Sentences
No. Mutations Sentence Comment
122 ABCC8 p.Ala284Lys
X
ABCC8 p.Ala284Lys 12471126:122:1216
status: NEW
view ABCC8 p.Ala284Lys details
 Alanine and lysine scanning of the Oxy273-285 peptide on DP401 and DP402 moleculesa Peptides Oxy273-285 Sequences KYFAATQFEPLAA 1 4 6 9 DP401 1 (Ϯ0) DP402 1 (Ϯ0) Oxy K273A AYFAATQFEPLAA 1 (Ϯ0.7) 1 (Ϯ0.3) Oxy Y274A KAFAATQFEPLAA 2 (Ϯ0.2) 2 (Ϯ0.5) Oxy F275A KYAAATQFEPLAA 40 (Ϯ25) 7 (Ϯ2.1) Oxy T278A KYFAAAQFEPLAA 1 (Ϯ0.2) 1 (Ϯ0.1) Oxy Q279A KYFAATAFEPLAA 1 (Ϯ0.7) 1 (Ϯ0.3) Oxy F280A KYFAATQAEPLAA 240 (Ϯ100) 75 (Ϯ31) Oxy E281A KYFAATQFAPLAA 0.5 (Ϯ0.3) 0.5 (Ϯ0) Oxy P282A KYFAATQFEALAA 2 (Ϯ0.2) 1 (Ϯ0.1) Oxy L283A KYFAATQFEPAAA 4 (Ϯ1.3) 4 (Ϯ1.3) Oxy Y274K KKFAATQFEPLAA 2 (Ϯ0.2) 3 (Ϯ0.1) Oxy F275K KYKAATQFEPLAA 400 (Ϯ270) 190 (Ϯ160) Oxy A276K KYFKATQFEPLAA 2 (Ϯ0.7) 2 (Ϯ0.2) Oxy A277K KYFAKTQFEPLAA 3 (Ϯ0.9) 2 (Ϯ0.5) Oxy T278K KYFAAKQFEPLAA 30 (Ϯ17) 5 (Ϯ0.4) Oxy Q279K KYFAATKFEPLAA 2 (Ϯ0.1) 1 (Ϯ0.1) Oxy F280K KYFAATQKEPLAA 270 (Ϯ180) 190 (Ϯ135) Oxy E281K KYFAATQFKPLAA 2 (Ϯ0.5) 1 (Ϯ0.2) Oxy P282K KYFAATQFEKLAA 2 (Ϯ1.2) 2 (Ϯ1.5) Oxy L283K KYFAATQFEPKAA 70 (Ϯ45) 80 (Ϯ37) Oxy A284K KYFAATQFEPLKA 2 (Ϯ0.9) 2 (Ϯ0.5) Oxy A285K KYFAATQFEPLAK 2 (Ϯ0.9) 2 (Ϯ0.7) a Capacity of the substituted peptides to bind HLA-DP401 and HLA-DP402 was investigated. Results are presented as relative activity (ratio between native and substituted peptide IC50). Login to comment