ABCMdb

PMID: 12404126

Ruefli AA, Tainton KM, Darcy PK, Smyth MJ, Johnstone RW
P-glycoprotein inhibits caspase-8 activation but not formation of the death inducing signal complex (disc) following Fas ligation.
Cell Death Differ. 2002 Nov;9(11):1266-72., [PubMed]

Sentences

No. Mutations Sentence Comment

109 ABCB1 p.Lys1076Met ABCB1 p.Lys1076Met ABCB1 p.Lys433Met ABCB1 p.Lys433Met The mutant P-gp used in the following studies contained double lysine to alanine substitutions at amino acids 233 (K433M) and 1076 (K1076M) within the Walker A motifs, which disrupted nucleotide binding, and ATPase and drug-efflux activity.4,16 Mutant constructs containing double lysine to alanine substitutions at 433(K433M) and 1076(K1076M) amino acids were generated by excising 4.1 kB Eag1 ± Eag1 fragment from clone pUCFVXMDH3 ± Sal(DAUG)-MDR/Neo (provided by Igor Roninson, Chicago University, Chicago, IL) and cloned into MSCV vector. Login to comment