ABCMdb

PMID: 12023951

Jungas T, Motta I, Duffieux F, Fanen P, Stoven V, Ojcius DM
Glutathione levels and BAX activation during apoptosis due to oxidative stress in cells expressing wild-type and mutant cystic fibrosis transmembrane conductance regulator.
J Biol Chem. 2002 Aug 2;277(31):27912-8. Epub 2002 May 22., 2002-08-02 [PubMed]

Sentences

No. Mutations Sentence Comment

42 ABCC7 p.Gly551Asp HeLa cells stably transfected with the plasmid alone (pTracer), the wild-type CFTR construct (pTCFwt), the CFTR ⌬F508 mutation (pTCF⌬F508), and the CFTR G551D mutation (pTCFG551D) were prepared as previously described (30). Login to comment 124 ABCC7 p.Gly551Asp The ability of H2O2 to activate BAX was then tested in pTracer, wild-type CFTR cells, ⌬F508, and cells expressing another CFTR mutation, G551D. Login to comment 125 ABCC7 p.Gly551Asp The G551D mutation is less common than ⌬F508, but it traffics normally to the plasma membrane, unlike ⌬F508, most of which is processed improperly and remains within the cell (44). Login to comment 162 ABCC7 p.Gly551Asp BAX activation in HeLa cells stably transfected with plasmid (black bars), wild-type CFTR (white bars), ⌬F508 CFTR (gray bars), or G551D CFTR (striped bars) was measured as a function of time after addition of 90 ␮M H2O2. Login to comment