ABCMdb

PMID: 12019078

Perea S, Lopez-Ribot JL, Wickes BL, Kirkpatrick WR, Dib OP, Bachmann SP, Keller SM, Martinez M, Patterson TF
Molecular mechanisms of fluconazole resistance in Candida dubliniensis isolates from human immunodeficiency virus-infected patients with oropharyngeal candidiasis.
Antimicrob Agents Chemother. 2002 Jun;46(6):1695-703., [PubMed]

Sentences

No. Mutations Sentence Comment

143 ABCB1 p.Val437Ala Four of these mutations (F126L, G307A, V437A, and G464S) have previously been described in azole-resistant C. albicans clinical isolates (16, 18, 19, 28, 36). Login to comment 150 ABCB1 p.Val437Ala ABCB1 p.Val125Leu V125L, F126L, and V437A mutations were found in ERG11 genes from both azole-susceptible and azole-resistant C. dubliniensis isolates and therefore are not likely to represent mutations linked with an phenotype of resistance to an azole antifungal agent. Login to comment 161 ABCB1 p.Thr199Ala ABCB1 p.Arg210Gly mutations (S113F, F148L, T199A, R210G, D288G, G289D, L300P, R398G, and P515S) have not been described in C. albicans. Login to comment 189 ABCB1 p.Val437Ala Four of these mutations (F126L, G307, V437A, and G464S) have been also found in clinical isolates of C. albicans with decreased susceptibilities to FLC, and two of them (G464S and G307A) have been shown to affect the affinity of the enzyme for the azoles. Login to comment 199 ABCB1 p.Val437Ala ABCB1 p.Thr199Ala ABCB1 p.Arg210Gly ABCB1 p.Ala595Gly ABCB1 p.Val125Ala ABCB1 p.Ala863Gly Nucleotide and amino acid substitutions in ERG11 genes from C. dubliniensis clinical isolates Patient no. Isolate FLC MIC (␮g/ml) Substitutiona S113F V125A F126L F148L T199A R210G D288G G289D L300P G307A R398G V437A G464S P515S 1 3300 1 T374C C378A T1310C 3946 64 C378A A595G A628G 4380 64 G1390A 4713 64 G1390A 4806 64 T442C G920C C1543T 4807 32 A863G T899C T1310C 5143 32 T374C C378A A1192G T1310C 2 3466 0.25 4572 64 C338T G866A a The base numbers are with respect to the first ATG codon of ERG11. Login to comment