PMID: 11893723

Flotte TR
Recombinant adeno-associated virus gene therapy for cystic fibrosis and alpha(1)-antitrypsin deficiency.
Chest. 2002 Mar;121(3 Suppl):98S-102S., [PubMed]
Sentences
No. Mutations Sentence Comment
24 ABCC7 p.Trp1282*
X
ABCC7 p.Trp1282* 11893723:24:140
status: NEW
view ABCC7 p.Trp1282* details
ABCC7 p.Trp1282*
X
ABCC7 p.Trp1282* 11893723:24:142
status: NEW
view ABCC7 p.Trp1282* details
 In Vitro Studies For in vitro studies of CFTR complementation, the immortalized CF bronchial epithelial cell line IB3-1 (genotype deltaF508/W1282X) was cultured in LHC-8E medium with 10% fetal bovine serum (37°C; 5% CO2).25 The constructs to be tested either were transfected using a reagent (Lipofectin; Invitrogen; Carlsbad, CA) or were infected at a multiplicity of infection ranging from 100 to 10,000 physical particles per cell. Login to comment