ABCMdb

PMID: 11864633

Bandi N, Kompella UB
Budesonide reduces multidrug resistance-associated protein 1 expression in an airway epithelial cell line (Calu-1).
Eur J Pharmacol. 2002 Feb 15;437(1-2):9-17., [PubMed]

Sentences

No. Mutations Sentence Comment

67 ABCC2 p.Leu23Arg The doxorubicin-resistant COR-L23R cells used as the MRP1 positive controls in the Western blot and vincristine sensitivity studies were N. Bandi, U.B. Kompella / European Journal of Pharmacology 437 (2002) 9-1710 obtained from Dr. Donald W. Miller (Nebraska Medical Center, Omaha, NE). Login to comment 81 ABCC2 p.Leu23Arg Cell lysates of the human lung carcinoma cells, COR-L23R, used as MRP1 positive controls (Thomas et al., 1994) in the Western blot analysis, were loaded at 10-mg protein. Login to comment 125 ABCC2 p.Leu23Arg In this study, MRP1 overexpressing COR-L23R cells seeded at densities of 5000 cells/well in 96-well plates were treated with various concentrations of vincristine (0.1-5 nM) with or without budesonide (10 mM). Login to comment 188 ABCC2 p.Leu23Arg * Indicates significant difference from controls in the accumulation and efflux studies. Fig. 6. Effect of the budesonide on the vincristine-mediated cytotoxicity in the COR-L23R cells. Login to comment 189 ABCC2 p.Leu23Arg The sensitivity of the COR-L23R cells to the vincristine was determined in the presence and absence of budesonide (10 mM) using an MTT assay. Login to comment 191 ABCC2 p.Leu23Arg * Indicates significant difference from controls in the accumulation and efflux studies. Fig. 6. Effect of the budesonide on the vincristine-mediated cytotoxicity in the COR-L23R cells. Login to comment 192 ABCC2 p.Leu23Arg The sensitivity of the COR-L23R cells to the vincristine was determined in the presence and absence of budesonide (10 mM) using an MTT assay. Login to comment 222 ABCC2 p.Leu23Arg In order to determine whether budesonide enhances the chemosensitivity of anticancer drugs, we determined the cytotoxic effects of vincristine with and without budesonide in COR-L23R cells. Login to comment 224 ABCC2 p.Leu23Arg The results of our study indicated that budesonide, at concentrations devoid of cytotoxicity (Table 1a), enhanced the sensitivity of COR-L23R to vincristine (Fig. 6), possibly by inhibiting MRP1 expression. Login to comment 225 ABCC2 p.Leu23Arg In order to determine whether budesonide enhances the chemosensitivity of anticancer drugs, we determined the cytotoxic effects of vincristine with and without budesonide in COR-L23R cells. Login to comment 226 ABCC2 p.Leu23Arg However, as the vincristine studies were performed in MRP1 overexpressing COR-L23R cells, we believe that our results are consistent with the inhibition of the MRP1-mediated efflux of vincristine. Login to comment 227 ABCC2 p.Leu23Arg The results of our study indicated that budesonide, at concentrations devoid of cytotoxicity (Table 1a), enhanced the sensitivity of COR-L23R to vincristine (Fig. 6), possibly by inhibiting MRP1 expression. Login to comment 229 ABCC2 p.Leu23Arg However, as the vincristine studies were performed in MRP1 overexpressing COR-L23R cells, we believe that our results are consistent with the inhibition of the MRP1-mediated efflux of vincristine. Login to comment