ABCMdb

PMID: 11546780

Conti LR, Radeke CM, Shyng SL, Vandenberg CA
Transmembrane topology of the sulfonylurea receptor SUR1.
J Biol Chem. 2001 Nov 2;276(44):41270-8. Epub 2001 Aug 23., [PubMed]

Sentences

No. Mutations Sentence Comment

55 ABCC8 p.Cys26Ser ABCC8 p.Cys170Ala The resulting SUR1AAA construct containing C6S, C26S, C170A, C1051S, and C1057S was referred to as NEC (no external cysteines). Login to comment 56 ABCC8 p.Thr99Cys ABCC8 p.Lys567Cys ABCC8 p.Tyr454Cys ABCC8 p.Lys337Cys NEC was subjected to PCR mutagenesis to individually introduce cysteines at each putative external loop (T99C, K337C, Y454C, K567C, T1161C, S1186C, and R1274C). Login to comment 57 ABCC8 p.Cys26Ser ABCC8 p.Cys170Ala The resulting SUR1AAA construct containing C6S, C26S, C170A, C1051S, and C1057S was referred to as NEC (no external cysteines). Login to comment 58 ABCC8 p.Thr99Cys ABCC8 p.Lys567Cys ABCC8 p.Tyr454Cys ABCC8 p.Lys337Cys NEC was subjected to PCR mutagenesis to individually introduce cysteines at each putative external loop (T99C, K337C, Y454C, K567C, T1161C, S1186C, and R1274C). Login to comment 165 ABCC8 p.Ala161Cys ABCC8 p.Arg388Cys Several of the mutations (A161C, R388C, P1162C, and R1300C) produced SUR1 proteins that did not show a complex glycosylated form, and these were not evaluated further. Login to comment 166 ABCC8 p.Ala161Cys ABCC8 p.Arg388Cys Several of the mutations (A161C, R388C, P1162C, and R1300C) produced SUR1 proteins that did not show a complex glycosylated form, and these were not evaluated further. Login to comment