ABCMdb

PMID: 11296221

Gaedeke N, Klein M, Kolukisaoglu U, Forestier C, Muller A, Ansorge M, Becker D, Mamnun Y, Kuchler K, Schulz B, Mueller-Roeber B, Martinoia E
The Arabidopsis thaliana ABC transporter AtMRP5 controls root development and stomata movement.
EMBO J. 2001 Apr 17;20(8):1875-87., [PubMed]

Sentences

No. Mutations Sentence Comment

60 ABCC7 p.Glu217Gly ATP-dependent uptake of oestradiol-17-(b-D-glucuronide) (E217G) and a rye ¯avonoid glucuronide was reported for vacuoles from rye and barley (Klein et al., 1998, 2000). Login to comment 63 ABCC7 p.Glu217Gly The yeast mutant transformed with AtMRP5 was able to transport E217G when compared with the empty vector control. Login to comment 65 ABCC7 p.Glu217Gly Reduced glutathione, oxidized glutathione and dinitrobenzene glutathione (DNB-GS) had no effect on E217G uptake. Login to comment 66 ABCC7 p.Glu217Gly However, the AtMRP5-dependent transport activity of E217G was severely in¯uenced by other organic anions such as oestradiol-3-sulfate, the natural ¯avone-glu-curonide luteolin-7-O-diglucuronide-4'-O-glucuronide, glycocholate and the sulfonylurea glibenclamide. Login to comment 109 ABCC7 p.Glu217Gly ABCC7 p.Glu217Gly Due to the variability of the uptake activities in different preparations, uptake rates were standardized to 100%, which corresponds to 7±25 pmol E217G/mg protein/min. Login to comment 206 ABCC7 p.Glu217Gly In addition to GS-Xs, AtMRP5 was also able to transport the model glucuronide E217G, a substance produced during the catabolism of steroids in animals but not known in plants. Login to comment 229 ABCC7 p.Glu217Gly The vacuolar transport rate for E217G and ¯avone glucuronides was strongly increased in the presence of the conjugate DNB-GS. Login to comment