ABCMdb

PMID: 11278980

Ahn W, Kim KH, Lee JA, Kim JY, Choi JY, Moe OW, Milgram SL, Muallem S, Lee MG
Regulatory interaction between the cystic fibrosis transmembrane conductance regulator and HCO3- salvage mechanisms in model systems and the mouse pancreatic duct.
J Biol Chem. 2001 May 18;276(20):17236-43. Epub 2001 Feb 22., 2001-05-18 [PubMed]

Sentences

No. Mutations Sentence Comment

60 ABCC7 p.Ser1455* Site-directed Mutagenesis-Oligonucleotide-directed mutagenesis using the GeneEditor mutagenesis kit (Promega, Madison, WI) was performed in the CFTR expression vector pCMVNot6.2 to delete the C-terminal 4 (⌬DTRL) or 26 (S1455X) amino acids. Login to comment 63 ABCC7 p.Ser1455* The mutagenesis primers were as follows: ⌬DTRL, 5Ј-GGA GAC AGA AGA AGA GGT GTA AGA TAC AAG GCT TTA GAG AG-3Ј; S1455X, 5Ј-GCT CTT TCC CCA CCG GAA CTG AAG CAA GTG CAA GTC TAA GCC-3Ј. Login to comment 174 ABCC7 p.Ser1455* Another finding of note in Fig. 5 is the behavior of a mutant CFTR protein lacking the final C-terminal 26 amino acids (S1455X). Login to comment 176 ABCC7 p.Ser1455* Similar to the findings with the CFTR-⌬DTRL, the CFTR-S1455X did not associate with NHE3 (Fig. 5B) and had no effect on NHE3 activity (Fig. 5C). Login to comment 208 ABCC7 p.Ser1455* PS120/NHE3 cells were transfected with mammalian expressing vectors for WT-CFTR, CFTR-⌬DTRL, and CFTR-S1455X. Login to comment