ABCMdb

PMID: 11121394

Takacs-Jarrett M, Sweeney WE, Avner ED, Cotton CU
Generation and phenotype of cell lines derived from CF and non-CF mice that carry the H-2K(b)-tsA58 transgene.
Am J Physiol Cell Physiol. 2001 Jan;280(1):C228-36., [PubMed]

Sentences

No. Mutations Sentence Comment

41 ABCC7 p.Ser489* The goal of this work was to cross the ImmortoMouse (26) with the University of North Carolina (UNC) CF knockout mouse (CFTR S489X) (40) and develop genetically well-matched, conditionally immortalized CF and non-CF epithelial cell lines. Login to comment 42 ABCC7 p.Ser489* METHODS Animals Male mice, homozygous for a temperature-sensitive SV40 large T antigen transgene (ImmortoMouse; CBA/ca X C57B1/10 strain; Charles River Laboratories) (26), were bred with female mice that were heterozygous for the S489X CFTR mutation (UNC; CFTR ϩ/-; C57BL/6J X F129 strain) (40). Login to comment 50 ABCC7 p.Ser489* Primers 5 and 6 were used to amplify the S489X neodisrupted allele of CFTR (NEO). Login to comment 145 ABCC7 p.Ser489* The CF cell lines (mTEC1-CF, mCT1-CF, mSEC1-CF, and mPEC1-CF) were negative for the wild-type CFTR allele (faint 200-bp bands are non-CFTR PCR products) and positive for the S489X neodisrupted allele of CFTR. Login to comment 151 ABCC7 p.Ser489* ABCC7 p.Ser489* Magnification, ϫ270. both the wild-type CFTR allele and the S489X neodisrupted allele, whereas one of the non-CF cell lines (mSEC1) was positive for the wild-type CFTR allele and negative for the S489X neodisrupted allele of CFTR. Login to comment 179 ABCC7 p.Ser489* Wild-type cystic fibrosis transmembrane conductance regulator (CFTR), neomycin disrupted S489X CFTR (NEO), and Immorto (IM) alleles were identified from PCR products separated by gel electrophoresis. Login to comment