ABCMdb

PMID: 11082117

Trapp S, Ashcroft FM
Direct interaction of Na-azide with the KATP channel.
Br J Pharmacol. 2000 Nov;131(6):1105-12., [PubMed]

Sentences

No. Mutations Sentence Comment

138 ABCC8 p.Lys719Ala To determine if the NBDs are also required for the ability of MgATP to support Na-azide activation we used a mutant SUR in which the conserved lysine in NBD1 was changed to alanine (SUR1-K719A). Login to comment 140 ABCC8 p.Lys719Ala Inhibition of Kir6.2-R50G/SUR1-K719A currents by 3 mM Na-azide amounted to 23+1% (n=3) in the absence of ATP, which was not signi®cantly dierent (P40.05) from that observed for Kir6.2-R50G/SUR1 currents (Figure 6C). Login to comment 141 ABCC8 p.Lys719Ala As previously reported (Gribble et al., 1998), 1 mM ATP did not activate Kir6.2-R50G/SUR1-K719A currents ± instead the current was blocked by 44+4% (n=6). Login to comment 177 ABCC8 p.Lys719Ala Na-azide (3 mM) and ATP (1 mM) were added to the internal solution as indicated by the bars. (B) Macroscopic Kir6.2-R50G/ SUR1-K719A currents recorded from an inside-out patch in response to voltage ramps from 7110 mV to +100 mV. Na-azide (3 mM) and ATP (1 mM) were added to the internal solution as indicated by the bars. (C) Mean slope conductance (G) in the presence of Na-azide or Na-azide plus ATP is expressed relative to the mean conductance in control solution (lacking Na-azide) before and after addition of the test solution (Gc). Login to comment