ABCMdb

PMID: 11034616

Aleksandrov AA, Chang X, Aleksandrov L, Riordan JR
The non-hydrolytic pathway of cystic fibrosis transmembrane conductance regulator ion channel gating.
J Physiol. 2000 Oct 15;528 Pt 2:259-65., 2000-10-15 [PubMed]

Sentences

No. Mutations Sentence Comment

64 ABCC7 p.Ser737Glu ABCC7 p.Ser795Glu ABCC7 p.Ser813Glu ABCC7 p.Ser660Glu 8SE construct The 8SE construct was made based on the 4SE mutant CFTR construct containing S660E, S737E, S795E and S813E (Chang et al. 1993). Login to comment 65 ABCC7 p.Ser700Glu The DraIII fragment containing 4SE was amplified by polymerase chain reaction (PCR) and an oligonucleotide primer to introduce the S700E mutation into the fragment. Login to comment 66 ABCC7 p.Ser712Glu ABCC7 p.Ser768Glu ABCC7 p.Ser686Glu This PCR product was used as a template for further amplification to introduce the S686E, S712E and S768E mutations. Login to comment 67 ABCC7 p.Ser795Glu ABCC7 p.Ser813Glu The DraIII fragment in the pNUT-4SE-CFTR was replaced by the DraIII fragment containing S660EÏS686EÏS700EÏS712EÏS737EÏS768E/S795E/S813E (8SE). Login to comment