ABCMdb

PMID: 11029305

Thomas EJ, Gabriel SE, Makhlina M, Hardy SP, Lethem MI
Expression of nucleotide-regulated Cl(-) currents in CF and normal mouse tracheal epithelial cell lines.
Am J Physiol Cell Physiol. 2000 Nov;279(5):C1578-86., [PubMed]

Sentences

No. Mutations Sentence Comment

20 ABCC7 p.Gly551Asp More recently, several transgenic mouse models of CF have been generated incorporating a knockout of CFTR or one of the CFTR mutations more commonly found in human populations (i.e., ⌬F508 or G551D) (12, 14, 15, 29, 33, 37, 39). Login to comment 42 ABCC7 p.Ser489* By use of this protocol, the CFTR knockout (S489X) gave a 210-bp product and the wild-type CFTR yielded a 190-bp product. PCR products were identified by electrophoresis on 2% (wt/vol) and 3% (wt/vol) agarose gels for TAg and CFTR products, respectively. Login to comment 90 ABCC7 p.Ser489* The CFTR and TAg genotype of each cell line was confirmed by PCR, which indicated that MTE18- (-/-) cells were homozygous for the S489X CFTR knockout, MTE7b-(ϩ/-) cells were heterozygous for this mutation (Fig. 1E), and both cell lines were positive for TAg (Fig. 1F). Login to comment 155 ABCC7 p.Ser489* In the present study, two such cell lines have been generated: one from a mouse homozygous for the S489X CFTR knockout and one from a mouse heterozygous for this mutation. Login to comment