ABCMdb

PMID: 10781583

Hou Y, Cui L, Riordan JR, Chang X
Allosteric interactions between the two non-equivalent nucleotide binding domains of multidrug resistance protein MRP1.
J Biol Chem. 2000 Jul 7;275(27):20280-7., 2000-07-07 [PubMed]

Sentences

No. Mutations Sentence Comment

33 ABCC1 p.Asp793Leu ABCC1 p.Asp1454Leu ABCC1 p.Asp792Leu ABCC1 p.Lys684Leu ABCC1 p.Glu1455Leu ABCC1 p.Lys1333Leu Stable cell lines expressing wild-type and mutant MRP1s, K684L, D792L/D793L, K1333L, and D1454L/E1455L, were generated by using procedures described previously (11). Login to comment 205 ABCC1 p.Asp1454Leu ABCC1 p.Lys684Leu ABCC1 p.Lys1333Leu Fig. 5C shows that although labeling by N3[␣-32 P]ATP was not abolished by the mutations, it was greatly reduced: K684L was ϳ10% of wild-type; K1333L, ϳ5%; D1454L/D1455L, ϳ15%. Login to comment 207 ABCC1 p.Asp1454Leu ABCC1 p.Lys684Leu ABCC1 p.Glu1455Leu ABCC1 p.Lys1333Leu Fig. 5D demonstrates that labeling of K684L by N3[␥-32 P]ATP was almost eliminated and labeling of K1333L and D1454L/E1455L were decreased to ϳ10% and ϳ15% of the wild-type levels, respectively. Login to comment 213 ABCC1 p.Lys684Leu ABCC1 p.Lys1333Leu The K1333L mutation in NBD2 nearly abolished ATP-dependent uptake as did the NBD2 Walker B substitution, whereas the K684L substitution reduced it by approximately half. Login to comment 232 ABCC1 p.Asp1454Leu ABCC1 p.Lys684Leu ABCC1 p.Glu1455Leu ABCC1 p.Lys1333Leu Lane 1, 10 ␮g of wild-type MRP1; lane 2, 20 ␮g of K684L; lane 3, 10 ␮g of K1333L; lane 4, 10 ␮g of D1454L/ E1455L. Login to comment 234 ABCC1 p.Asp1454Leu ABCC1 p.Lys684Leu ABCC1 p.Glu1455Leu ABCC1 p.Lys1333Leu Lane 1, 10 ␮g of wild-type MRP1; lane 2, 20 ␮g of K684L; lane 3, 10 ␮g of K1333L; lane 4, 10 ␮g of D1454L/ E1455L. Login to comment 235 ABCC1 p.Asp1454Leu ABCC1 p.Lys684Leu ABCC1 p.Glu1455Leu ABCC1 p.Lys1333Leu E, ATP-dependent LTC4 uptake by membrane vesicles containing wild-type (closed diamonds) and mutant MRPs: NBD1 Walker A lysine mutant K684L (open circles), NBD2 Walker A lysine mutant K1333L (open square), NBD2 Walker B aspartate mutant D1454L/ E1455L (closed circles). Login to comment