ABCMdb

PMID: 10777731

Enkvetchakul D, Loussouarn G, Makhina E, Shyng SL, Nichols CG
The kinetic and physical basis of K(ATP) channel gating: toward a unified molecular understanding.
Biophys J. 2000 May;78(5):2334-48., [PubMed]

Sentences

No. Mutations Sentence Comment

51 ABCC8 p.Ala161Cys ABCC8 p.Ile162Cys Patches were exposed to differing [ATP] as indicated Table 1 K1/2,ATP and Pozero values (estimated from noise analysis or single channels) for mutant Kir6.2 ؉ SUR1 channels examined in this study (L164, R176 mutants), in Shyng et al. (1997a, N160 mutants) and in Loussouarn et al. (2000, Cysteine substitutions) Pozero Ϯ SE mean K1/2,ATP (␮M) Ϯ SE WT 0.45 Ϯ 0.09 12 Ϯ 1.6 L164T 0.89 Ϯ 0.01 4,300 L164G 0.89 Ϯ 0.03 3,600 L164A 0.87 Ϯ 0.02 520 L164V 0.78 Ϯ 0.04 7 R176A 0.01 Ϯ 0.001 5 N160D 0.83 Ϯ 0.01 46 N160A 0.76 Ϯ 0.02 6 N160E 0.83 Ϯ 0.03 18 Ctrl 0.88 Ϯ 0.02 1,060 Ϯ 10 Ctrl N/D 0.88 Ϯ 0.01 1,060 Ϯ 24 Ctrl-N153C 0.88 Ϯ 0.02 230 Ϯ 76 Ctrl-I154C 0.89 Ϯ 0.01 330 Ϯ 30 Ctrl-L157C N/D 0.89 Ϯ 0.01 420 Ϯ 270 Ctrl-M158C N/D 0.89 Ϯ 0.02 4,200 Ϯ 1300 Ctrl-A161C N/D 0.88 Ϯ 0.01 110 Ϯ 8 Ctrl-I162C N/D 0.86 Ϯ 0.03 33 Ϯ 6 Ctrl-M163C N/D 0.90 Ϯ 0.02 190 Ϯ 54 Ctrl-L164C N/D 0.89 Ϯ 0.01 Ͼ100,000 Ctrl-I167C N/D 0.88 Ϯ 0.02 1,100 Ϯ 250 Ctrl-M169C N/D 0.90 Ϯ 0.01 110 Ϯ 17 Ctrl-T171C 0.91 Ϯ 0.02 2,800 Ϯ 820 Ctrl-Q173C 0.88 Ϯ 0.01 33 Ϯ 4 Ctrl-H175C N/D 0.90 Ϯ 0.03 24,500 Ϯ 4970 All channels were coexpressed with SUR1 subunits. Login to comment