PMID: 10694816

Licht T, Goldenberg SK, Vieira WD, Gottesman MM, Pastan I
Drug selection of MDR1-transduced hematopoietic cells ex vivo increases transgene expression and chemoresistance in reconstituted bone marrow in mice.
Gene Ther. 2000 Feb;7(4):348-58., [PubMed]
Sentences
No. Mutations Sentence Comment
175 ABCB1 p.Gly185Val
X
ABCB1 p.Gly185Val 10694816:175:280
status: NEW
view ABCB1 p.Gly185Val details
 Materials and methods Generation and titration of retroviral producer cells Construction of plasmid pHaMDR1/A which consists of a full-length human MDR1 cDNA flanked by long-terminal repeats from Harvey sarcoma virus has been reported.52 This MDR1 cDNA contains a point mutation (G185V) which confers preferential resistance to colchicine.45,46 By transfection of pHaMDR1/A into PA317 packaging cells, a virus producing cell clone, designated MA1, was generated. Login to comment