ABCMdb

PMID: 10381582

Reimann F, Tucker SJ, Proks P, Ashcroft FM
Involvement of the n-terminus of Kir6.2 in coupling to the sulphonylurea receptor.
J Physiol. 1999 Jul 15;518 ( Pt 2):325-36., [PubMed]

Sentences

No. Mutations Sentence Comment

25 ABCC8 p.Lys719Ala ABCC8 p.Lys719Ala However, when the Pï was decreased (by ATP), tolbutamide was unable to block Kir6.2ÄN14ÏSUR1-K719A,K1385M currents, despite the fact that the drug inhibited Kir6.2-C166SÏSUR1-K719A,K1385M currents (which in the absence of ATP have a Pï of > 0·8 and are not blocked by tolbutamide). Login to comment 46 ABCC8 p.Lys719Ala We use the abbreviation SUR1-KAKM to refer to mutation of the Walker A lysines in both NBD1 (K719A) and NBD2 (K1385M) of SUR1. Login to comment 169 ABCC8 p.Lys719Ala Figure 9Ab shows that the ability of tolbutamide to block the channel in the presence of MgATP was also abolished when both Walker A lysines were mutated (SUR1-K719A,K1385M, abbreviated SUR1-KAKM). Login to comment