ABCMdb

PMID: 10096895

Yamazaki J, Britton F, Collier ML, Horowitz B, Hume JR
Regulation of recombinant cardiac cystic fibrosis transmembrane conductance regulator chloride channels by protein kinase C.
Biophys J. 1999 Apr;76(4):1972-87., [PubMed]

Sentences

No. Mutations Sentence Comment

34 ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala Site-directed mutagenesis The serine at position 686 and/or 790 was modified by polymerase chain reaction-based site-directed mutagenesis (Jones and Howard, 1991) to alanine to create S686A, S790A, and S686 ϩ 790A cardiac CFTR cDNA. Login to comment 281 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala ABCC7 p.Ser790Ala We examined cAMP and PDBu regulation of cardiac (exon 5afa;) CFTR channels in oocytes injected with mRNA encoding three mutant constructs: S686A, S790A, and the double mutant S686A-S790A. Login to comment 282 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala Fig. 10, A and B, shows representative currents at 70 mV for the S686A and S686A-S790A mutants during exposure to the cAMP cocktail, PDBu, and then a subsequent cAMP cocktail in the continued presence of PDBu. Login to comment 283 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala ABCC7 p.Ser790Ala We examined cAMP and PDBu regulation of cardiac (exon 5-) CFTR channels in oocytes injected with mRNA encoding three mutant constructs: S686A, S790A, and the double mutant S686A-S790A. Login to comment 284 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala Fig. 10, A and B, shows representative currents at 70 mV for the S686A and S686A-S790A mutants during exposure to the cAMP cocktail, PDBu, and then a subsequent cAMP cocktail in the continued presence of PDBu. Login to comment 285 ABCC7 p.Ser686Ala Fig. 10 C shows a comparison of the absolute current amplitudes activated by cAMP in Xenopus oocytes injected with 47 ng of mRNA encoding S686A(card), S790A(card), S686A-S790A(card), wild-type cardiac (card), or wild-type epithelial CFTR. Login to comment 287 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala Fig. 10 C shows a comparison of the absolute current amplitudes activated by cAMP in Xenopus oocytes injected with 47 ng of mRNA encoding S686A(card), S790A(card), S686A-S790A(card), wild-type cardiac (card), or wild-type epithelial CFTR. Login to comment 289 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala ABCC7 p.Ser790Ala This is consistent with a previous report that showed no significant differences in PKA activation sensitivity for the S686A mutant in epithelial CFTR channels (Wilkinson et al., 1996). Login to comment 291 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala ABCC7 p.Ser790Ala Normalized PDBu-induced current amplitudes were 55.2 Ϯ 8.8% (n ϭ 4) for wild-type channels, 27.6 Ϯ 5.5% (n ϭ 5) for S686A, 29.0 Ϯ 3.6% (n ϭ 4) for S790A, and 25.1 Ϯ 2.4% (n ϭ 4) for the S686A-S790A double mutant. Login to comment 313 ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala However, in oo- FIGURE 10 Effects of cAMP and PDBu on cardiac S686A and S790A mutant CFTR channels. Login to comment 314 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala (A and B) The effects of cAMP cocktail (1afb;) and PDBu (100 nM) on cardiac CFTR-S686A and CFTR-S686A, S790A were examined using the same protocol as in Fig. 5 A. Login to comment 315 ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala However, in oo- FIGURE 10 Effects of cAMP and PDBu on cardiac S686A and S790A mutant CFTR channels. Login to comment 316 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala (A and B) The effects of cAMP cocktail (1ϫ) and PDBu (100 nM) on cardiac CFTR-S686A and CFTR-S686A, S790A were examined using the same protocol as in Fig. 5 A. Login to comment 337 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala ABCC7 p.Ser790Ala PDBu-induced CFTR current amplitudes were reduced by approximately half in the S686A, S790A, and the double mutant S686A-S790A constructs examined. Login to comment 339 ABCC7 p.Ser686Ala ABCC7 p.Ser686Ala ABCC7 p.Ser790Ala ABCC7 p.Ser790Ala PDBu-induced CFTR current amplitudes were reduced by approximately half in the S686A, S790A, and the double mutant S686A-S790A constructs examined. Login to comment