ABCA3 p.Pro72Arg
| Predicted by SNAP2: | A: N (53%), C: D (53%), D: N (57%), E: N (53%), F: D (71%), G: D (59%), H: N (53%), I: D (63%), K: N (53%), L: D (66%), M: D (63%), N: N (57%), Q: N (61%), R: D (53%), S: N (57%), T: N (61%), V: D (53%), W: D (80%), Y: D (63%), |
| Predicted by PROVEAN: | A: N, C: D, D: D, E: N, F: N, G: D, H: N, I: N, K: N, L: N, M: N, N: N, Q: N, R: N, S: N, T: N, V: N, W: D, Y: N, |
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[hide] Factors involved in the cisplatin resistance of KC... Oncol Rep. 2014 Feb;31(2):719-26. doi: 10.3892/or.2013.2896. Epub 2013 Dec 5. Oiso S, Takayama Y, Nakazaki R, Matsunaga N, Motooka C, Yamamura A, Ikeda R, Nakamura K, Takeda Y, Kariyazono H
Factors involved in the cisplatin resistance of KCP4 human epidermoid carcinoma cells.
Oncol Rep. 2014 Feb;31(2):719-26. doi: 10.3892/or.2013.2896. Epub 2013 Dec 5., [PMID:24317338]
Abstract [show]
KCP-4 is a cisplatin-resistant cell line established from human epidermoid carcinoma KB-3-1 cells. Although our previous study revealed that one of the mechanisms for cisplatin resistance in KCP-4 cells is the activation of NF-kappaB, its high resistance is considered to be induced by multiple mechanisms. In the present study, we explored other factors involved in the development of cisplatin resistance in KCP-4 cells. Since it has been reported that an unknown efflux pump exports cisplatin from KCP-4 cells in an ATP-dependent manner, we examined 48 types of ATP-binding cassette proteins as candidate cisplatin efflux transporters. The mRNA expression levels of ABCA1, ABCA3, ABCA7 and ABCB10 in KCP-4 cells were higher when compared to those in KB-3-1 cells. These expression levels in cisplatin-sensitive revertant KCP-4 cells (KCP-4R cells), were reduced in parallel with the sensitivity of these cells to cisplatin and their intracellular accumulation of cisplatin. Next, we investigated the occurrence of mutations in p53 in KCP-4 cells. We found a heterozygous missense mutation at codon 72 (p.Pro72Arg) in p53 of both KCP-4 and KB-3-1 cells, but the protein expression level of p53 in KCP-4 cells was higher when compared to that in KB-3-1. These results suggest that ABCA1, ABCA3, ABCA7 and ABCB10 are candidate genes for the cisplatin efflux transporter that is involved in the cisplatin resistance of KCP-4 cells, and that the mutation at codon 72 of p53 may contribute to the development of cisplatin resistance.
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7 Next, we investigated the occurrence of mutations in p53 in KCP-4 cells. We found a heterozygous missense mutation at codon 72 (p.Pro72Arg) in p53 of both KCP-4 and KB-3-1 cells, but the protein expression level of p53 in KCP-4 cells was higher when compared to that in KB-3-1.
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ABCA3 p.Pro72Arg 24317338:7:130
status: NEW