ABCC2 p.Thr1286Cys
| Predicted by SNAP2: | A: N (87%), C: N (87%), D: N (93%), E: N (93%), F: N (82%), G: N (93%), H: N (93%), I: N (82%), K: N (93%), L: N (82%), M: N (87%), N: N (93%), P: N (93%), Q: N (97%), R: N (87%), S: N (97%), V: N (87%), W: N (53%), Y: N (78%), |
| Predicted by PROVEAN: | A: N, C: N, D: N, E: N, F: D, G: D, H: N, I: N, K: N, L: N, M: N, N: N, P: N, Q: N, R: N, S: N, V: N, W: D, Y: N, |
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[hide] Allelic-specific expression in relation to Bombyx ... Insect Biochem Mol Biol. 2014 Nov;54:53-60. doi: 10.1016/j.ibmb.2014.07.007. Epub 2014 Aug 12. Chen Y, Li M, Islam I, You L, Wang Y, Li Z, Ling L, Zeng B, Xu J, Huang Y, Tan A
Allelic-specific expression in relation to Bombyx mori resistance to Bt toxin.
Insect Biochem Mol Biol. 2014 Nov;54:53-60. doi: 10.1016/j.ibmb.2014.07.007. Epub 2014 Aug 12., [PMID:25123097]
Abstract [show]
Understanding the mechanism of Bt resistance is one of the key elements of the effective application of Bt in pest control. The lepidopteran model insect, the silkworm, demonstrates qualities that make it an ideal species to use in achieving this understanding. We screened 45 strains of silkworm (Bombyx mori) using a Cry1Ab toxin variant. The sensitivity levels of the strains varied over a wide range. A resistant strain (P50) and a phylogenetically related susceptible strain (Dazao) were selected to profile the expressions of 12 Bt resistance-related genes. The SNPs in these genes were detected based on EST analysis and were validated by allelic-specific PCR. A comparison of allelic-specific expression between P50 and Dazao showed that the transcript levels of heterozygous genes containing two alleles rather than an imbalanced allelic expression contribute more to the resistance of P50 against Bt. The responses of the allelic-specific expression to Bt in hybrid larvae were then investigated. The results showed that the gene expression pattern of an ATP-binding cassette transporter C2 (ABCC2) and an aminopeptidase N (APN3), changed in an allelic-specific manner, with the increase of the resistant allele expression correlated with larval survival. The results suggest that a trans-regulatory mechanism in ABCC2 and APN3 allelic-specific expression is involved in the insect's response to the Bt toxin. The potential role of allelic-specific gene regulation in insect resistance to Bt toxins is discussed.
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No. Sentence Comment
127 Seven (Bt175 C4887A, AA T1286C, APN1 A1464*, APN1 T1626A, APN1 C2227A, APN3 T2420G, and APN4 T2658G) were heterozygous, with two alleles expressed at the same time.
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ABCC2 p.Thr1286Cys 25123097:127:24
status: NEW