ABCB6 p.Pro72Arg
| Predicted by SNAP2: | A: N (66%), C: N (61%), D: D (63%), E: D (63%), F: N (57%), G: N (53%), H: N (57%), I: N (66%), K: D (59%), L: N (93%), M: N (82%), N: N (53%), Q: N (53%), R: D (59%), S: N (61%), T: N (57%), V: N (66%), W: D (59%), Y: N (57%), |
| Predicted by PROVEAN: | A: N, C: N, D: N, E: N, F: N, G: N, H: N, I: N, K: N, L: N, M: N, N: N, Q: N, R: N, S: N, T: N, V: N, W: N, Y: N, |
[switch to compact view]
Comments [show]
None has been submitted yet.
[hide] Factors involved in the cisplatin resistance of KC... Oncol Rep. 2014 Feb;31(2):719-26. doi: 10.3892/or.2013.2896. Epub 2013 Dec 5. Oiso S, Takayama Y, Nakazaki R, Matsunaga N, Motooka C, Yamamura A, Ikeda R, Nakamura K, Takeda Y, Kariyazono H
Factors involved in the cisplatin resistance of KCP4 human epidermoid carcinoma cells.
Oncol Rep. 2014 Feb;31(2):719-26. doi: 10.3892/or.2013.2896. Epub 2013 Dec 5., [PMID:24317338]
Abstract [show]
KCP-4 is a cisplatin-resistant cell line established from human epidermoid carcinoma KB-3-1 cells. Although our previous study revealed that one of the mechanisms for cisplatin resistance in KCP-4 cells is the activation of NF-kappaB, its high resistance is considered to be induced by multiple mechanisms. In the present study, we explored other factors involved in the development of cisplatin resistance in KCP-4 cells. Since it has been reported that an unknown efflux pump exports cisplatin from KCP-4 cells in an ATP-dependent manner, we examined 48 types of ATP-binding cassette proteins as candidate cisplatin efflux transporters. The mRNA expression levels of ABCA1, ABCA3, ABCA7 and ABCB10 in KCP-4 cells were higher when compared to those in KB-3-1 cells. These expression levels in cisplatin-sensitive revertant KCP-4 cells (KCP-4R cells), were reduced in parallel with the sensitivity of these cells to cisplatin and their intracellular accumulation of cisplatin. Next, we investigated the occurrence of mutations in p53 in KCP-4 cells. We found a heterozygous missense mutation at codon 72 (p.Pro72Arg) in p53 of both KCP-4 and KB-3-1 cells, but the protein expression level of p53 in KCP-4 cells was higher when compared to that in KB-3-1. These results suggest that ABCA1, ABCA3, ABCA7 and ABCB10 are candidate genes for the cisplatin efflux transporter that is involved in the cisplatin resistance of KCP-4 cells, and that the mutation at codon 72 of p53 may contribute to the development of cisplatin resistance.
Comments [show]
None has been submitted yet.
No. Sentence Comment
116 This missense mutation results in the substitution of proline at codon 72 for arginine (p.Pro72Arg).
X
ABCB6 p.Pro72Arg 24317338:116:90
status: NEW