ABCB1 p.Glu275Ala
| Predicted by SNAP2: | A: N (82%), C: N (61%), D: N (93%), F: N (72%), G: N (78%), H: N (66%), I: N (72%), K: N (87%), L: N (72%), M: N (61%), N: N (87%), P: D (66%), Q: N (93%), R: N (82%), S: N (82%), T: N (87%), V: N (78%), W: D (53%), Y: N (53%), |
| Predicted by PROVEAN: | A: D, C: D, D: N, F: D, G: D, H: D, I: D, K: N, L: D, M: D, N: D, P: D, Q: N, R: N, S: D, T: D, V: D, W: D, Y: D, |
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[hide] A salt bridge in intracellular loop 2 is essential... Biochemistry. 2013 May 14;52(19):3194-6. doi: 10.1021/bi400425k. Epub 2013 May 3. Loo TW, Clarke DM
A salt bridge in intracellular loop 2 is essential for folding of human p-glycoprotein.
Biochemistry. 2013 May 14;52(19):3194-6. doi: 10.1021/bi400425k. Epub 2013 May 3., [PMID:23634976]
Abstract [show]
There is no high-resolution structure of the human P-glycoprotein (P-gp, ABCB1) drug pump. Homology models based on the crystal structures of mouse and Caenorhabditis elegans P-gps show extensive contacts between intracellular loop 2 (ICL2, in the first transmembrane domain) and the second nucleotide-binding domain. Human P-gp modeled on these P-gp structures yields different ICL2 structures. Only the model based on the C. elegans P-gp structure predicts the presence of a salt bridge. We show that the Glu256-Arg276 salt bridge was critical for P-gp folding.
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No. Sentence Comment
31 It was found that the E255A, K271A, K272A, E273A, and E275A mutants were different from the E256A and R276A mutants because they yielded mature 170 kDa P-gp as their major product (Figure 1E).
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ABCB1 p.Glu275Ala 23634976:31:54
status: NEW