ABCMdb

ABCD3 p.Ile307Ala

Predicted by SNAP2:	A: N (61%), C: N (72%), D: D (71%), E: D (71%), F: N (78%), G: D (71%), H: D (66%), K: D (71%), L: N (93%), M: N (93%), N: D (53%), P: D (75%), Q: N (72%), R: D (75%), S: D (53%), T: D (59%), V: N (78%), W: D (66%), Y: N (78%),
Predicted by PROVEAN:	A: D, C: D, D: D, E: D, F: N, G: D, H: D, K: D, L: N, M: N, N: D, P: D, Q: D, R: D, S: D, T: D, V: N, W: D, Y: D,

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[hide] Hydrophobic regions adjacent to transmembrane doma... J Biol Chem. 2007 Nov 16;282(46):33831-44. Epub 2007 Aug 30. Kashiwayama Y, Asahina K, Morita M, Imanaka T
Hydrophobic regions adjacent to transmembrane domains 1 and 5 are important for the targeting of the 70-kDa peroxisomal membrane protein.
J Biol Chem. 2007 Nov 16;282(46):33831-44. Epub 2007 Aug 30., [PMID:17761678]

Abstract [show]
The 70-kDa peroxisomal membrane protein (PMP70) is a major component of peroxisomal membranes. Human PMP70 consists of 659 amino acid residues and has six putative transmembrane domains (TMDs). PMP70 is synthesized on cytoplasmic ribosomes and targeted posttranslationally to peroxisomes by an unidentified peroxisomal membrane protein targeting signal (mPTS). In this study, to examine the mPTS within PMP70 precisely, we expressed various COOH-terminally or NH(2)-terminally deleted constructs of PMP70 fused with green fluorescent protein (GFP) in Chinese hamster ovary cells and determined their intracellular localization by immunofluorescence. In the COOH-terminally truncated PMP70, PMP70(AA.1-144)-GFP, including TMD1 and TMD2 of PMP70, was still localized to peroxisomes. However, by further removal of TMD2, PMP70(AA.1-124)-GFP lost the targeting ability, and PMP70(TMD2)-GFP did not target to peroxisomes by itself. The substitution of TMD2 in PMP70(AA.1-144)-GFP for TMD4 or TMD6 did not affect the peroxisomal localization, suggesting that PMP70(AA.1-124) contains the mPTS and an additional TMD is required for the insertion into the peroxisomal membrane. In the NH(2)-terminal 124-amino acid region, PMP70 possesses hydrophobic segments in the region adjacent to TMD1. By the disruption of these hydrophobic motifs by the mutation of L21Q/L22Q/L23Q or I70N/L71Q, PMP70(AA.1-144)-GFP lost targeting efficiency. The NH(2)-terminally truncated PMP70, GFP-PMP70(AA.263-375), including TMD5 and TMD6, exhibited the peroxisomal localization. PMP70(AA.263-375) also possesses hydrophobic residues (Ile(307)/Leu(308)) in the region adjacent to TMD5, which were important for targeting. These results suggest that PMP70 possesses two distinct targeting signals, and hydrophobic regions adjacent to the first TMD of each region are important for targeting.

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243 Peroxisome Targeting Signal of PMP70 NOVEMBER 16, 2007•VOLUME 282•NUMBER 46 JOURNAL OF BIOLOGICAL CHEMISTRY 33841 acid pair existing just adjacent to TMD5 was important for the targeting to peroxisomes; GFP-PMP70(AA.263-375 I307A/L308A) was directed to peroxisomes, but GFP-PMP70(AA.263-375 I307N/L308Q) was not (Fig. 8). Login to comment
244 Peroxisome Targeting Signal of PMP70 NOVEMBER 16, 2007ߦVOLUME 282ߦNUMBER 46 JOURNAL OF BIOLOGICAL CHEMISTRY 33841 acid pair existing just adjacent to TMD5 was important for the targeting to peroxisomes; GFP-PMP70(AA.263-375 I307A/L308A) was directed to peroxisomes, but GFP-PMP70(AA.263-375 I307N/L308Q) was not (Fig. 8). Login to comment