ABCC8 p.Leu511Pro
| Predicted by SNAP2: | A: N (53%), C: N (82%), D: D (75%), E: D (66%), F: N (72%), G: D (66%), H: D (63%), I: N (61%), K: D (71%), M: N (82%), N: D (66%), P: D (75%), Q: D (59%), R: D (66%), S: N (53%), T: D (53%), V: N (61%), W: D (59%), Y: N (53%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: D, G: D, H: D, I: N, K: D, M: N, N: D, P: D, Q: D, R: D, S: D, T: D, V: D, W: D, Y: D, |
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[hide] Performance of MTBDR plus for detecting high/low l... Int J Tuberc Lung Dis. 2009 Feb;13(2):260-5. Brossier F, Veziris N, Jarlier V, Sougakoff W
Performance of MTBDR plus for detecting high/low levels of Mycobacterium tuberculosis resistance to isoniazid.
Int J Tuberc Lung Dis. 2009 Feb;13(2):260-5., [PMID:19146757]
Abstract [show]
SETTING: We recently evaluated the Genotype MTBDR test for assessing Mycobacterium tuberculosis resistance to rifampicin (RMP) and isoniazid (INH) by detecting mutations in rpoB (codons 511-533) and katG (codon 315). A new version of the test, MTBDR plus, has been designed to also detect mutations in the regulatory region of inhA. OBJECTIVE: To evaluate the performance of MTBDR plus over MTBDR. RESULTS: In 113 isolates, MTBDR plus detected all 76 RMP-resistant (RMP-R) strains and all 64 INH-resistant (INH-R) strains with KatG-315 mutations, 59 of which displayed a high level of INH resistance. It also identified 18 strains undetectable by MTBDR, without mutation in KatG-315 but with a -15 C-->T mutation in the regulatory region of inhA, of which 15 displayed a low level of INH resistance. Thirteen INH-R strains, which mainly harboured mutations in KatG at positions other than 315, were undetected by MTBDR plus. CONCLUSION: MTBDR plus retains the accuracy shown by MTBDR in detecting RMP resistance and is more sensitive in detecting INH resistance (86% vs. 67%), particularly at low levels (minimum inhibitory concentration<1 mg/l, 69% vs. 17%). The negative predictive value of the test (the probability of a strain with a wild-type test being susceptible to INH) is >98% when the rate of INH is <10%, as it is in France.
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No. Sentence Comment
27 One of the RMP-resistant strains found by sequencing to carry three mutations, F505L, L511P and S531C, yielded negative hybridisation signals with the wild-type probes WT1 (covering position 505),WT2 (position 511) and WT8 (position 531).
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ABCC8 p.Leu511Pro 19146757:27:86
status: NEW