ABCA1 p.Gln270Arg
| Predicted by SNAP2: | A: N (87%), C: N (87%), D: N (93%), E: N (97%), F: N (66%), G: N (82%), H: N (87%), I: N (82%), K: N (97%), L: N (87%), M: N (93%), N: N (97%), P: N (87%), R: N (93%), S: N (97%), T: N (93%), V: N (93%), W: D (59%), Y: N (57%), |
| Predicted by PROVEAN: | A: D, C: D, D: N, E: N, F: D, G: D, H: N, I: D, K: N, L: D, M: N, N: N, P: D, R: N, S: N, T: D, V: D, W: D, Y: D, |
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[hide] Phospholipid transfer protein interacts with and s... J Biol Chem. 2003 Dec 26;278(52):52379-85. Epub 2003 Oct 14. Oram JF, Wolfbauer G, Vaughan AM, Tang C, Albers JJ
Phospholipid transfer protein interacts with and stabilizes ATP-binding cassette transporter A1 and enhances cholesterol efflux from cells.
J Biol Chem. 2003 Dec 26;278(52):52379-85. Epub 2003 Oct 14., [PMID:14559902]
Abstract [show]
Phospholipid lipid transfer protein (PLTP) is ubiquitously expressed in animal tissues and plays multiple roles in lipoprotein metabolism, but the function of peripheral PLTP is still poorly understood. Here we show that one of its possible functions is to transport cholesterol and phospholipids from cells to lipoprotein particles by a process involving PLTP interactions with cellular ATP-binding cassette transporter A1 (ABCA1). When ABCA1 was induced in murine macrophages or ABCA1-transfected baby hamster kidney cells, PLTP gained the ability to promote cholesterol and phospholipid efflux from cells. Although PLTP alone had lipid efflux activity, its maximum activity was observed in the presence of high density lipoprotein particles. Pulsechase studies showed that the interaction of PLTP with ABCA1-expressing cells played a role in promoting lipid efflux. Overexpression of ABCA1 dramatically increased binding of both PLTP and apoA-I to common sites on the cell surface. Both PLTP and apoA-I were covalently cross-linked to ABCA1, each protein blocked cross-linking of the other, and both PLTP and apoA-I stabilized ABCA1 protein. These results are consistent with PLTP and apoA-I binding to ABCA1 at the same or closely related sites. Thus, PLTP mimics apolipoproteins in removing cellular lipids by the ABCA1 pathway, except that PLTP acts more as an intermediary in the transfer of cellular lipids to lipoprotein particles.
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No. Sentence Comment
41 Printed in U.S.A. This paper is available on line at http://www.jbc.org L89P, Q270R, and S387P) as described previously (24).
X
ABCA1 p.Gln270Arg 14559902:41:80
status: NEW40 52, Issue of December 26, pp. 52379-52385, 2003 (c) 2003 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in U.S.A. This paper is available on line at http://www.jbc.org L89P, Q270R, and S387P) as described previously (24).
X
ABCA1 p.Gln270Arg 14559902:40:206
status: NEW