ABCC8 p.Thr1130Ile
| Predicted by SNAP2: | A: N (78%), C: N (57%), D: N (53%), E: N (53%), F: D (66%), G: N (61%), H: D (63%), I: N (66%), K: D (53%), L: N (66%), M: D (53%), N: N (78%), P: D (59%), Q: N (72%), R: N (53%), S: N (87%), V: N (66%), W: D (53%), Y: D (66%), |
| Predicted by PROVEAN: | A: N, C: N, D: D, E: N, F: N, G: D, H: D, I: N, K: N, L: N, M: N, N: N, P: D, Q: N, R: N, S: N, V: N, W: N, Y: N, |
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[hide] Identification of the high-affinity tolbutamide si... Diabetes. 1999 Jun;48(6):1341-7. Ashfield R, Gribble FM, Ashcroft SJ, Ashcroft FM
Identification of the high-affinity tolbutamide site on the SUR1 subunit of the K(ATP) channel.
Diabetes. 1999 Jun;48(6):1341-7., [PMID:10342826]
Abstract [show]
ATP-sensitive potassium channels (K(ATP)) are formed from four pore-forming Kir6.2 subunits complexed with four regulatory sulfonylurea receptor subunits (SUR1 in pancreatic beta-cells, SUR2A in heart). The sensitivity of the channel to different sulfonylureas depends on the SUR isoform. In particular, Kir6.2-SUR1 but not Kir6.2-SUR2A channels are blocked by tolbutamide with high affinity. We made chimeras between SUR1 and SUR2A to identify the region of the protein involved in high-affinity tolbutamide block. Chimeric SURs were coexpressed with Kir6.2 in Xenopus oocytes, and macroscopic currents were measured in inside-out membrane patches. High-affinity tolbutamide inhibition could be conferred on SUR2A by replacing transmembrane domains (TMs) 14-16 with the corresponding region of SUR1. Conversely, high-affinity tolbutamide inhibition of SUR1 was abolished by replacing TMs 13-16 with the corresponding SUR2A sequence, or by mutating a single serine residue within this region to tyrosine (S1237Y). Binding of [3H]glibenclamide to membranes expressing SUR1 was abolished concomitantly with the loss of high-affinity tolbutamide block. These results suggest that a site in the COOH-terminal set of TMs of the SUR1 subunit of the K(ATP) channel is involved in the binding of tolbutamide and glibenclamide.
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No. Sentence Comment
127 Most mutations did not affect tolbutamide sensitivity: these included C1128T, T1130I, C1141S, C1174F, Q1190E, S1201C, A1204S, Y1218H, Q1223K, Y1229L, L1226M (data not shown; n = 2-4 patches in each case).
X
ABCC8 p.Thr1130Ile 10342826:127:78
status: NEW