ABCC8 p.Ala30Met
Predicted by SNAP2: | C: N (57%), D: D (66%), E: D (66%), F: D (66%), G: N (87%), H: D (63%), I: N (78%), K: N (57%), L: D (59%), M: D (59%), N: D (59%), P: N (53%), Q: D (59%), R: D (63%), S: N (93%), T: N (93%), V: N (78%), W: D (53%), Y: D (66%), |
Predicted by PROVEAN: | C: N, D: D, E: D, F: D, G: N, H: D, I: N, K: N, L: N, M: N, N: D, P: D, Q: N, R: N, S: N, T: N, V: N, W: D, Y: D, |
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[hide] Conformational changes of pediocin in an aqueous m... Int J Biol Macromol. 2003 Mar;32(1-2):1-9. Gaussier H, Lavoie M, Subirade M
Conformational changes of pediocin in an aqueous medium monitored by fourier transform infrared spectroscopy: a biological implication.
Int J Biol Macromol. 2003 Mar;32(1-2):1-9., [PMID:12719125]
Abstract [show]
Fourier transform infrared (FTIR) spectroscopy was used to investigate the secondary structure of pediocin PA-1 in different aqueous media in relation to its antimicrobial activity. The experiments were performed at pD (pH meter corrected for deuterium isotope effect) 6, 7, and 8 and during a heating-cooling cycle of 20-80 degrees C. At pD 6, (i.e. pediocin's most active form), the FTIR results show that pediocin adopts an unordered structure with a small contribution of beta-turn. After a heating-cooling cycle, thermally-induced changes in pediocin are reversed and its activity is maintained. Increasing the pD to 7 and 8 leads to a more ordered secondary structure. For these two pD values, an increase in temperature induces an irreversible aggregation of protein as revealed by the amide I' band. The analysis of the Tyr region provides more insight into the aggregation process. In fact, it appears to be a two-step process, involving first the C (carboxy)-terminus of pediocin and then the N (amino)-terminus. This study reveals two major points: (1) the preservation of pediocin flexibility is essential for maintaining its activity; and (2) the aggregation of its C-terminus is sufficient to induce a loss of activity, suggesting that this region plays an important role in the activity of pediocin.
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No. Sentence Comment
25 Its primary structure has been determined by Edman degradation [7] and it consists of 44 amino acids: NH2-Lys-Tyr-Tyr-Gly-Asn5-Gly-Val-Thr-Cys-Gly10-Lys- His-Ser-Cys-Ser15-Val-Asp-Trp-Gly-Lys20-Ala-Thr-Thr- Cys-Ile25-Ile-Asn-Asn-Gly-Ala30-Met-Ala-Trp-Ala-Thr35- Gly-Gly-His-Gln-Gly40-Asn-His-Lys-Cys-COOH.
X
ABCC8 p.Ala30Met 12719125:25:233
status: NEW