ABCC8 p.Asn72Ala
| Predicted by SNAP2: | A: N (66%), C: N (61%), D: N (53%), E: N (53%), F: N (66%), G: N (78%), H: N (87%), I: N (57%), K: N (57%), L: N (66%), M: N (57%), P: D (59%), Q: N (66%), R: N (66%), S: N (78%), T: N (82%), V: N (66%), W: D (59%), Y: N (78%), |
| Predicted by PROVEAN: | A: D, C: D, D: N, E: N, F: D, G: N, H: N, I: D, K: D, L: D, M: D, P: D, Q: N, R: D, S: N, T: N, V: D, W: D, Y: D, |
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[hide] Structural inhibition of the colicin D tRNase by t... EMBO J. 2004 Apr 7;23(7):1474-82. Epub 2004 Mar 11. Graille M, Mora L, Buckingham RH, van Tilbeurgh H, de Zamaroczy M
Structural inhibition of the colicin D tRNase by the tRNA-mimicking immunity protein.
EMBO J. 2004 Apr 7;23(7):1474-82. Epub 2004 Mar 11., [PMID:15014439]
Abstract [show]
Colicins are toxins secreted by Escherichia coli in order to kill their competitors. Colicin D is a 75 kDa protein that consists of a translocation domain, a receptor-binding domain and a cytotoxic domain, which specifically cleaves the anticodon loop of all four tRNA(Arg) isoacceptors, thereby inactivating protein synthesis and leading to cell death. Here we report the 2.0 A resolution crystal structure of the complex between the toxic domain and its immunity protein ImmD. Neither component shows structural homology to known RNases or their inhibitors. In contrast to other characterized colicin nuclease-Imm complexes, the colicin D active site pocket is completely blocked by ImmD, which, by bringing a negatively charged cluster in opposition to a positively charged cluster on the surface of colicin D, appears to mimic the tRNA substrate backbone. Site-directed mutations affecting either the catalytic domain or the ImmD protein have led to the identification of the residues vital for catalytic activity and for the tight colicin D/ImmD interaction that inhibits colicin D toxicity and tRNase catalytic activity.
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No. Sentence Comment
106 The most prominent feature is a high degree of charge Table II Site-directed mutagenesisa Mutated ColD residue In vivob In vitroc Mutated ImmD residue In vivob In vitroc colD+ImmD ÀImmD +ImmD colD+ImmD colD+ImmD wt ColD + + À wt ImmD + 2 L595P + + À K11A + À S601P + + À L12A + À R602G + + À S16A + À K603E + + À K17A + À D606H + + À E22A + À K607A +/22 +/22 À R26A + À K608A 2 2 ND E30A + À K610A 2 2 ND R31A + + H611A 2 2 ND R32A + À D614A +/22 + +/À R32Q + +/2 Y649A +/2 +/2 À R33A + À R651A 2 2 ND Y35A + À E652A + + À N46A + +/2 K670A + + À E49A + +/22 S677A 2 2 ND F52A + + W679A 2 2 ND M53A + À K680A + + À E56A + +/2 N682A +/2 +/2 À R57A + +/ÀÀ A685Q +/2 + +/À E59A + + D686A + +/2 2 D61A + À D63A + À Y67A + À E68A + À N72A + +/2 K75A + + V76A + +/2 R79A + + E83A + +/2 K84A + +/2 Substitutions of functionally important residues are indicated by bold characters.
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ABCC8 p.Asn72Ala 15014439:106:888
status: NEW